Replies to post #334082 on Anavex Life Sciences Corp (AVXL)

[Investor2014]

One of the several reasons that I pay less attention to anything publised that involves Tanzi. Think Prana, Deepak Chopra association and involment alongside his wife with all sorts of pseudo science.

In my review I paid most attention to the last sentence of the paragraph I quoted in my post.

The significant effects of S1R-activation or -inactivation on MAM-IP3R3 levels as opposed to the more modest effects on total-IP3R3 suggest S1R preferentially modulates MAMs in FAD hNPCs

That sentence supports the consensus understanding from numerous prior papers on agonising and increasing the expression of S1R.

Also refer to this quote from the paper (related to introducing NE-100):

We performed the CytoTox-ONE assay to measure lactate dehydrogenase (LDH) levels in the culture media to assess cell viability after introducing si-S1R for 0, 48, and 72 h. We observed ~77% reduction of S1R expression after 48 h, and >90% reduction after 72 h transfection with si-S1R. The LDH-assay revealed that 72 h transfection with si-S1R led to ~53% loss of cell viability, whereas 48 h transfection exhibited little or no reduction in cell viability (Figure S2).