Priorities US202062995717P·2020-02-11; US202117172818A·2021-02-10 Application US202117172818A·2021-02-10 Publication US2021246471A1·2021-08-12 Published as US2021246471A1;WO2021163249A1
EN MODIFICATION OF HEAVY CHAIN FIBROIN IN BOMBYX MORI
Abstract Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with the heavy chain fibroin protein. Embodiments include insertion and truncation vectors utilized for modifying the FibH gene. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into the FibH gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing an enhanced silk product with a high percentage of spider silk proteins. Embodiments include a silk product produced by such transgenic Bombyx mori.