Friday, August 27, 2021 7:29:49 AM
KRAIG BIOCRAFT LABORATORIES INC [US]
Inventors
KANE TREVOR LOGAN [US]; LICON ANA LAURA [US]; THOMPSON KIMBERLY KRAIG [US]
Classifications
IPC
A01K67/04; C07K14/435; C12N15/11; C12N15/90; C12N9/22;
CPC
A01K67/04 (US); C07K14/43518 (US); C12N15/11 (US); C12N15/902 (US); C12N9/22 (US); A01K2217/054 (US); A01K2217/075 (US); A01K2227/706 (US); A01K2267/01 (US); C12N2310/20 (US); C12N2800/80 (US);
Priorities
US202062995717P·2020-02-11; US202117172818A·2021-02-10
Application
US202117172818A·2021-02-10
Publication
US2021246471A1·2021-08-12
Published as
US2021246471A1;WO2021163249A1
EN
MODIFICATION OF HEAVY CHAIN FIBROIN IN BOMBYX MORI
Abstract
Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with the heavy chain fibroin protein. Embodiments include insertion and truncation vectors utilized for modifying the FibH gene. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into the FibH gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing an enhanced silk product with a high percentage of spider silk proteins. Embodiments include a silk product produced by such transgenic Bombyx mori.
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