Replies to post #330531 on NorthWest Biotherapeutics Inc (NWBO)

[Evaluate]

Some of the other Patent info is interesting too, on the same link
https://patents.justia.com/assignee/northwest-biotherapeutics-inc

For example:
OPTIMALLY ACTIVATED DENDRITIC CELLS THAT INDUCE AN IMPROVED OR INCREASED ANTI-TUMOR IMMUNE RESPONSE
Publication number: 20180187145
includes:

Abstract: The present disclosure provides populations of cells comprising partially mature and optimally activated dendritic cells that can be used for administration to individuals having a cancer and/or tumor.
Partially matured dendritic cells, those contacted with a dendritic cell maturation agent for about 10 to about 19 hours, upon administration efficiently take up and process tumor antigens in the area of the tumor site, complete maturation, and can subsequently migrate to the lymph nodes of a treated individual.
Once in a lymph node the now fully mature antigen presenting dendritic cells secrete the appropriate cytokines (e.g., TNF?, IL-6, IL-8, and/or IL-12) and contact T cells inducing a substantial and optimal clinical and/or anti-tumor immune response.

I seems like we heard about the optimal activation time for DC's ... looks to me that 10 to 19 hours might be "optimal".
(Although this 2012 patent appears to indicate 1 to 10 hours, or more:

ADMINISTRATION OF DENDRITIC CELLS PARTIALLY MATURED IN VITRO FOR THE TREATMENT OF TUMORS
Publication number: 20120251561

And remember the TFF devices? I wonder if they might work nicely together with the Flaskworks devices?

Tangential flow filtration devices and methods for leukocyte enrichment
Patent number: 8518636
Abstract: The present invention provides tangential flow filtration devices and methods for enriching a heterogenous mixture of blood constituents for leukocytes by removal of non-leukocyte blood constituents. In one particular embodiment the device can provide a composition enriched in monocytes.
Type: Grant
Filed: April 13, 2010
Date of Patent: August 27, 2013