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bow-tie

09/23/20 11:24 AM

#20284 RE: Staypositive1 #20283

nice friend nice find!! that's nice news
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Work Harder

09/23/20 12:00 PM

#20289 RE: Staypositive1 #20283

Thanks for that one also

b/c we both know who is in

Nottingham

:}
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Work Harder

09/23/20 2:53 PM

#20299 RE: Staypositive1 #20283

University Hospital Inselspital, Berne

Collaborator: Boehringer Ingelheim
Abbott
Merck Sharp & Dohme Corp.
Solvay Pharmaceuticals

https://ichgcp.net/clinical-trials-registry/NCT00783380

March 2008

Phase 4

Virosomal influenza vaccine

Influvacplus 2005/2006, Solvay Pharma AG, Bern, Switzerland Assigned to all 4 groups if immunocompromized patients

https://clinicaltrials.gov/ct2/history/NCT00783380?V_2=View
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Phosphene

09/23/20 4:05 PM

#20308 RE: Staypositive1 #20283

Gratitude to you for sharing and Dr. Bottazzi for tweeting.
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Work Harder

09/24/20 9:42 AM

#20314 RE: Staypositive1 #20283

Monophosphoryl Lipid A-Adjuvanted Virosomes with Ni-Chelating Lipids for Attachment of Conserved Viral Proteins as Cross-Protective Influenza Vaccine

Dec 26 2017

Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB Groningen, The Netherlands

Recombinant pET32a-NP and pET32a-EGFP plasmids were generated by inserting the NP gene derived from A/Hongkong/2/1968 (H3N2, called HK68 in the following) or the gene encoding enhanced green fluorescent protein (EGFP) into vector pET32a (Merck Millipore, Germany). The recombinant plasmids were transfected into competent Escherichia coli AD494 (Merck Millipore) and the cells were cultured in LB broth supplemented with ampicillin and kanamycin. When an OD600 of 0.8 was reached, 0.5?mM isopropyl ß-d-1-thiogalactopyranoside (IPTG) was added to induce the expression of the his-tagged proteins. His-tagged proteins were purified by Ni-chelate affinity resin (Merck Millipore). Briefly, BugBuster protein extraction reagent (Merck Millipore) with 6?M urea was used for lysis of transfected E. coli. Ni-NTA resin was equilibrated with binding buffer (0.1?M HEPES, 0.5?M NaCl) and then incubated with E. coli lysate at 4?°C overnight with rotation.

https://onlinelibrary.wiley.com/doi/full/10.1002/biot.201700645

Matrix-M Adjuvated Seasonal Virosomal Influenza Vaccine Induces Partial Protection in Mice and Ferrets against Avian H5 and H7 Challenge

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581625/

Tween-20 (Merck Millipore
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Work Harder

09/24/20 10:12 AM

#20315 RE: Staypositive1 #20283

This is coming around friend

https://investorshub.advfn.com/boards/read_msg.aspx?message_id=153673753

It's going to turn some

THIS YEAR

IMHO
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Work Harder

09/24/20 2:55 PM

#20347 RE: Staypositive1 #20283

Air quotes

Additional preclinical studies at other sites will be announced in the coming weeks.

& Vincent we all know you are still gathering funding

LOL