Mymetics Corp (MYMX)

[Work Harder]

Monophosphoryl Lipid A-Adjuvanted Virosomes with Ni-Chelating Lipids for Attachment of Conserved Viral Proteins as Cross-Protective Influenza Vaccine

Dec 26 2017

Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB Groningen, The Netherlands

Recombinant pET32a-NP and pET32a-EGFP plasmids were generated by inserting the NP gene derived from A/Hongkong/2/1968 (H3N2, called HK68 in the following) or the gene encoding enhanced green fluorescent protein (EGFP) into vector pET32a (Merck Millipore, Germany). The recombinant plasmids were transfected into competent Escherichia coli AD494 (Merck Millipore) and the cells were cultured in LB broth supplemented with ampicillin and kanamycin. When an OD600 of 0.8 was reached, 0.5?mM isopropyl ß-d-1-thiogalactopyranoside (IPTG) was added to induce the expression of the his-tagged proteins. His-tagged proteins were purified by Ni-chelate affinity resin (Merck Millipore). Briefly, BugBuster protein extraction reagent (Merck Millipore) with 6?M urea was used for lysis of transfected E. coli. Ni-NTA resin was equilibrated with binding buffer (0.1?M HEPES, 0.5?M NaCl) and then incubated with E. coli lysate at 4?°C overnight with rotation.

https://onlinelibrary.wiley.com/doi/full/10.1002/biot.201700645

Matrix-M Adjuvated Seasonal Virosomal Influenza Vaccine Induces Partial Protection in Mice and Ferrets against Avian H5 and H7 Challenge

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581625/

Tween-20 (Merck Millipore