Replies to post #248890 on NorthWest Biotherapeutics Inc (NWBO)

[sukus]

Lykiri thanks for the article. I just read this 5-ala article. Now I understand better what this technique can do. In my limited understanding, it made so much sense to me. Besides helping during the surgery by helping surgeons recognize the tumor better through different coloration of the tumor, it also gives additional benefits by causing tumor antigens be easily picked up by Dendritic Cells. Of course I believe anything that can make Dendritic cells better able to readily pick up tumor antigens would increase the effectiveness of using this therapy.

[flipper44]

Yes, I read that a while back. Thanks.

Putting many articles together, I come to this conclusion.

The beauty of DCVax-L, where the lysate portion is made using freeze thaw, made from a tumor that was resected using 5-ala, is that they stimulate a synergistic immune response.

5-ala resection

1. increases DAMPS (Danger signals),
2. Immunogenicity/antigen expression
3. Reduces remaining tumor microenvironment interference in the body by decreasing the number of cancer cells remaining after surgery via improved visualization and some apoptosis (which also increases immune response).
4. Increases antigen availability for lysate.

Yet all these things as a montherapy do not result in significant survival advantage.

However, then take that 5-ala resected tumor, and put it through the freeze thaw process, then

Combining it with naive DCVax dendritic cells and you:

1. Further increase DAMPS with freeze thaw process.
2. Reduce tumor microenvironment interference by dendritic cell antigen uptake outside the immunosuppressed body where (local and systemic).
3. Create highly costimulated mature dendritic cells that upon reinfection into the body will not be influenced by systemic immunosuppressive environment.
4. Express to a broader spectrum of t-cells without inducing toleration.
5. T-cells are fired up and ready to go against more targets, plus innate system is also placed on higher alert.
6. The first true immunogenic response after DC administration is the most important, and it is critical that it not be one that allows some toleration of the cancer cells -- therefore the steps above help insure this.