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jondoeuk

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jondoeuk

Re: jondoeuk post# 107

Sunday, 05/07/2023 2:52:55 PM

Sunday, May 07, 2023 2:52:55 PM

Post# of 151
Some more info. Pre-activation is IL-12/15/18 and expansion with uAPCs (engineered K562 feeder cells expressing mbIL-21 and 4-1BBL) plus IL-2, transduction with a retroviral vector, before another round of expansion. From two cord blood units, 250 doses have been manufactured with a (CAR) transduction efficiency of 60%. Six dose levels will be tested, with DL1 completed. Also, over 90 cryopreservation protocols were tested, with MDACC outperforming standard frozen.
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