Anavex Life Sciences Corp (AVXL)

[Investor2014]

The paper found that overdoing antagonism with NE-100 eventually lead cells losing viablity, -53% after 72 hours after exposure. I would think overdoing agnonism or any unnatural levels of cell exposure to anything will eventually be very harmful.

To study the effect of disrupted MAMs on the processing and ß-secretase cleavage of palAPP, we silenced the expression of S1R in FAD hNPCs, using a SMART pool small interfering RNA (siRNA) against sigmar1 (si-S1R) (Amer et al., 2013). We performed the CytoTox-ONE assay to measure lactate dehydrogenase (LDH) levels in the culture media to assess cell viability after introducing si-S1R for 0, 48, and 72 h. We observed ~77% reduction of S1R expression after 48 h, and >90% reduction after 72 h transfection with si-S1R. The LDH-assay revealed that 72 h transfection with si-S1R led to ~53% loss of cell viability, whereas 48 h transfection exhibited little or no reduction in cell viability (Figure S2).