Tornado Alley (PROG)

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The Evolution of Bacteria on a “Mega-Plate” Petri Dish

Published on Sep 9, 2016 by Harvard Medical School [ http://www.youtube.com/channel/UCjG-bUhM813LndEDNOJNCcw / http://www.youtube.com/user/harvardmedicalschool , http://www.youtube.com/user/harvardmedicalschool/videos ]

In a creative stroke inspired by Hollywood wizardry, scientists have designed a simple way to observe how bacteria move as they become impervious to drugs. The experiments are thought to provide the first large-scale glimpse of the maneuvers of bacteria as they encounter increasingly higher doses of antibiotics and adapt to survive—and thrive—in them.

Read the full story at http://hms.harvard.edu/news/bugs-screen

http://www.youtube.com/watch?v=plVk4NVIUh8 [with comments]


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Evolution of antibiotic resistance in real time in a 2'x4' petri dish

Published on Sep 8, 2016 by Michael Baym [ http://www.youtube.com/channel/UCaIHGpknqCOIiQYrzeWQNTw / http://www.youtube.com/user/mhbaym , http://www.youtube.com/user/mhbaym/videos ]

Bacteria (white) grow up to the boundary where they can no longer survive. Mutants, capable of surviving the higher concentration of antibiotic appear and invade the new band. Subsequent steps require further mutations. After about 11 days, resistance to over 1000 times as much antibiotic as was originally toxic evolves.

This appears as movie S1 from: http://science.sciencemag.org/content/353/6304/1147
by Michael Baym, Tami D. Lieberman, Eric D. Kelsic, Remy Chait, Rotem Gross, Idan Yelin, and Roy Kishony.

The MEGA-plate with an exponential trimethoprim gradient (0-3-30-300-3000-300-30-3-0 MIC).

This movie was compiled from time-lapse imagery every 10 minutes for 11.7 days, and played at 30fps (18000X speed). Each second of video is approximately five hours of real time.

Condensation on the lid is visible in the first several frames, and a single contaminating colony appears on the plate.

http://www.youtube.com/watch?v=GHI45garS3g [comments disabled]


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Evolution of ciprofloxacin resistance in E. Coli

Published on Sep 8, 2016 by Michael Baym

Four-step Ciprofloxacin MEGA-plate, movie S3 from http://science.sciencemag.org/content/353/6304/1147

The MEGA-plate with a CPR gradient as in fig. S1 (0-20-200-2000-20000-2000-200-20-0). Movie was compiled from time-lapse imagery every 10 minutes for 14.2 days, and played at 30fps (18000X speed).

http://www.youtube.com/watch?v=I6Pl9Lg_m-4 [comments disabled]


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A Gorgeous—and Unsettling—Video of Evolution in Action

09.09.16
https://www.wired.com/2016/09/gorgeous-unsettling-video-evolution-action/ [with embedded video; no comments yet]

Stunning Videos of Evolution in Action

The MEGA-plate allows scientists to watch bacteria adapting to antibiotics before their eyes.
Sep 8, 2016
http://www.theatlantic.com/science/archive/2016/09/stunning-videos-of-evolution-in-action/499136/ [with embedded videos, and comments]

WATCH: Bacteria Invade Antibiotics And Transform Into Superbugs
September 8, 2016
http://www.npr.org/sections/health-shots/2016/09/08/492965889/watch-bacteria-invade-antibiotics-and-transform-into-superbugs [with embedded video]

Watch as Bacteria Evolve Antibiotic Resistance in a Gigantic Petri Dish

9/08/16
http://gizmodo.com/watch-as-bacteria-evolve-antibiotic-resistance-in-a-gig-1786389688 [with embedded video, and comments]


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