Replies to post #20301 on NanoViricides Inc (NNVC)

[westeffer]

My eyes need EKC....I didn't see the "additional results".

[kulnor]

Looks like this is the last presentation today at the conference. Anyone by any chance attending?
http://www.gtcbio.com/userAgenda.aspx?id=148

1:45 Effect of Topical Nanoviricides on Rabbit Model of EKC
Vivien Boniuk, M.D., Associate Professor, Department of Ophthalmology & Visual
Sciences, Albert Einstein Medical Center

PURPOSE: To Evaluate Effects of Topical Nanoviricides on Rabbit Model of EKC METHODS: Utilizing EKC rabbit model described by YJ Gordon et al,12 female New Zealand Rabbits were inoculated topically and intrastromally with Adenovirus Type 5, under the operating microscope under anesthesia. All 12 rabbits had procedures performed on both eyesrabbit # 12 received BSS instead of virus. After 15 hours all animals except Group D received 2 drops to both eyes 50ul per drop twice a day for 10 days in a blinded manner. Group D received no drops at all. There were 12 rabbits in 4 groups: GroupA vehicle control Group B NNVC B Group C NNVC C and Group D. Swabs were taken pre-inoculatiomn, at 3 hours after viral adsorption and at days 3,6,9, and 21 as well as at sacrifice Day 37. Blood samples were taken pre-inoculation and Days 9, 21, and 37, spun down and frozen at -70 degrees. Rabbits were monitored daily 1-10 and days 21 and 37 graded 0-4 for conjunctival injection, blepharitis and corneal subepithelial infilatrates by 2 observers. RESULTS: There were higher clinical scores for conjunctival injection and blepharitis in Group A than in Groups NNVC B and NNVC C. The two infected untreated rabbits in Group D were similar to Group A. The sham infected rabbit in Group D had mild initial reaction, did not develop clinical disease and had no SEI''s. Conjunctivitis and blepharitis scores were still elevated in Group A at Day 10, but in both B & C had returned to baseline. SEI''s were not detected until Day 21 in a few instances. By Day 37 sacrifice 83% of eyes in Control Group A had SEI''s, none of NNVCB Group and 50% of NNVC C Group had SEI''s. In D Group, 100% of untreated eyes had SEI''s and 0/2 of the sham-inoculated eyes had SEI''s. CONCLUSIONS: In rabbit experimental model of EKC, NNVC B showed significant effect in mitigating clinical and immune response of EKC and NNVC C showed lesser effect.