ExcellGene sells purified trimeric Spike protein preparations from CHO cells to commercial companies for internal research and for use in diagnostic applications.
For CHO-based production of a trimeric Spike protein the construct Spike_?Cter_?Furin_2P_T4_His was used53. Briefly, based on the CHO-vector pXLG-6 (ExcellGene SA), containing a puromycin resistance marker and optimized expression elements, the variant spike sequence was inserted, containing a scambled furin cleavage site sequence, a two-prolin sequence for blocking the protein in the prefusion form, a 3’ T4-trimerization DNA, followed by a hexahistidin tag sequence.
From chemical transfection (CHO4Tx®, ExcellGene) to clonal selection and isolation of protein producing cell lines, to subsequent expansion of cells and production – all steps were done under suspension culture in chemically defined media with strict adherence to regulatory recommendations.
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