Replies to post #22113 on Mymetics Corp (MYMX)

[Work Harder]

Viral Clearance Kits

https://www.cygnustechnologies.com/browse/viral-clearance-kits?gclid=EAIaIQobChMIr7nVpd_p7QIVg4bACh07xwJOEAAYASAAEgIQTPD_BwE

Maravai LifeSciences acquired MockV Solutions to further strengthen its position in bioprocess impurity analytics with products for viral clearance testing. MockV Solutions has been integrated into Cygnus operations.

https://www.cygnustechnologies.com/about-us

https://www.maravai.com/portfolio

& the reply to

https://investorshub.advfn.com/boards/read_msg.aspx?message_id=158151881

und

https://investorshub.advfn.com/boards/read_msg.aspx?message_id=158274119

Yah, I'd say Maria is eager

https://loop.frontiersin.org/people/366565/overview

:}

[bow-tie]

Pseudotyped Lentivirus Vectors - Baylor College of Medicine
www.bcm.edu/research/research-services/service...
Pseudotypes prepared using VSV-G, LCMV, and RRV envelope proteins make the vector less inflammatory with tropism for the liver (Park, 2003) (Kang et al., 2002), while those prepared using Ebola or Marburg virus coat protein allow transduction of apical surface airway epithelium (Kobinger et al., 2001).

https://www.bcm.edu/research/research-services/service-labs/vector-development/lentivirus-vectors/pseudotyped-lentivirus-vectors


Abstract
Virosomes as membranous vesicles with viral fusion protein in their membrane are versatile vehicles for cargo delivery. The vesicular stomatitis virus glycoprotein (VSV-G) is a common fusogenic protein used in virosome preparation. This glycoprotein has been used in liposomal systems so far, but in this study, we have tried to use the niosomal form instead of liposome for. Niosomes are vesicular systems composed of non-ionic surfactants. Niosomes were constructed by the thin-film hydration method. VSV-G gene in pMD2.G plasmid was expressed in the HEK293T cell line and then was reconstituted in the niosome bilayer. The formation of niosomal virosomes was confirmed with different methods such as SDS-PAGE gel, western blotting, and transmission electron microscopy (TEM). The efficiency of niosomal virosome was investigated with the pmCherry reporter gene. SDS-PAGE and western blotting proved the expression and successful insertion of protein into the bilayer. The TEM images showed the spike projection of VSV-G on the surface of niosomes. The transfection results showed high efficiency of niosomal virosomes as a novel carrier. This report has verified that niosome could be used as an efficient bilayer instead of liposome to construct virosomes.

Keywords: Gene delivery; Liposome; Niosome; Protein reconstitution; VSV-G; Virosome.

Copyright © 2020 Elsevier Inc. All rights reserved.

https://www.science.gov/scigov/desktop/en/results.html


T7-driven tetracistronic Ebola virus trVLP system with a Renilla-luciferase reporter (pT7.1-4cis-EBOV-vRNA-hrLuc, pTH5264), recombinant non-infectious virus system for Ebola virus Mayinga (8 Plasmids)

https://www.european-virus-archive.com/nucleic-acid/t7-driven-tetracistronic-ebola-virus-trvlp-system-renilla-luciferase-reporter-pt71-4cis

https://www.european-virus-archive.com/partners