Table 2B
Prevalence, sensitivity, specificity, positive, and negative predictive values after the
addition of bacitracin to all washing steps
Percent (95% CI)
Prevalence 31.3 (24.2–39.5)
Sensitivity 100 (89.1–100)
Specificity 93.6 (86.9–97.2)
Positive predictive value 85.1 (71.1–93.3)
Negative predictive value 100 (95.5–100)
We have found that by adding bacitracin to each washing step, one
may greatly reduce interference with Enterococcus. While this allowed
for the increased specificity found when samples were retested, it
should be noted that there were still 7 samples that were positive by
the rapid test but negative by traditional culture. While it is possible
that these samples may have additional contaminants causing the
results to be persistently falsely positive, it may be possible that a GBS
non–beta-hemolytic variant would be missed by traditional culture
but detected by the antibody-based rapid test. Indeed, one of these
samples was confirmed to contain GBS by PCR.
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