Biotech Values

[jellybean]

pharmacia and upjohn

United States Patent 5,721,114
Abrahamsen , et al. February 24, 1998

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Expression system for producing apolipoprotein AI-M


Abstract
The invention relates to an expression system giving high extracellular production of apolipoprotein AI-M (Milano) using E. coli and comprises a plasmid carrying an origin of replication, an inducible promoter sequence, a DNA sequence coding for a signal peptide, a DNA sequence coding for apolipoprotein AI-M, and a transcription terminator. The invention also relates to a method of producing apolipoprotein AI-M using the expression system.


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Inventors: Abrahamsen; Lars (Stockholm, SE), Holmgren; Erik (Lidingo, SE), Kalderen; Christina (Stockholm, SE), Lake; Mats (Lidingo, SE), Mikaelsson; .ANG.sa (Stockholm, SE), Sejlitz; Torsten (Solna, SE)
Assignee: Pharmacia & Upjohn Aktiebolag (Stockholm, SE)