Rather than relying on one fixed enzyme like Bxb1 (SGMO's MINT platform), Seamless engineers multiple recombinase families, including large serine recombinases and others like Cre, and uses directed evolution plus machine learning (to tailor them to target chosen sequences). This potentially allows the enzymes designed by Seamless to excise, replace, invert, or insert DNA, giving broader (functional) capabilities beyond just integration. The goal is direct, precise gene insertions without the need for genomic landing pads.
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