Mymetics Corp (MYMX)

[Work Harder]

Qb phage protein

Structures of Qß virions, virus-like particles, and the Qß–MurA complex reveal internal coat proteins and the mechanism of host lysis

Biology, Baylor College of Medicine, Houston, TX, 77030

o build the atomic model for the Qß VLP capsid, our previous icosahedral Qß structure (PDB ID: 5KIP) was used as the initial model and subsequently refined into the 3.3-Å icosahedral map of the Qß VLP by the real-space refinement from PHENIX (53). To build the backbone model for the Qß virion capsid, the crystal structure of A2 (PDB ID: 5MNT) was used to replace one coat protein dimer on the Qß VLP capsid from the previous step. The model for the internalized coat protein dimer was then added to the capsid of the Qß virion by fitting into its corresponding density as a rigid body. The backbone of the Qß virion capsid was then refined into the 4.4-Å asymmetric map using the real-space refinement with the secondary structure restraints in PHENIX. To model the interaction between A2 and MurA, the density of the A2–MurA complex was firstly segmented out from the 5.7-Å map of the Qß–MurA complex using University of California San Francisco (UCSF) Chimera (54). The crystal structures of MurA (PDB ID: 1UAE) and A2 were then rigidly fitted into the segmented density and further refined by the molecular dynamics flexible fitting method (55) followed by the real-space refinement with the secondary structure restraints in PHENIX. The model statistics are summarized in SI Appendix, Table S1.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676892/

Production and characterization of novel ssRNA bacteriophage virus-like particles from metagenomic sequencing data

Riga, LV1067 Latvia

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6513524/

Particle-Based Platforms for Malaria Vaccines

The Qb VLP has also been used as a core to present multiple copies
of recombinant Pfs25 on its surface. Immunizing mice with the
Pfs25-Q VLP, formulated with or without Alhydrogel®, induced
anti-Pfs25 antibody titers higher than that of Pfs25-EPA conjugates. The antibodies displayed transmission blocking activity, and
stronger IgG2 and IgG3

Yimin Wua,*, David L. Naruma, Sylvain Fleury b, Gary Jennings c,1, Anjali Yadava d

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4688205/