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Monday, 11/26/2018 2:38:48 PM

Monday, November 26, 2018 2:38:48 PM

Post# of 27661
Jane Spirkoski
Molecular and Cell Biologist
The Hague Area, Netherlands

Mymetics Corp. Oct 2016 to Present. 2YRS 2MOS. Leiden, South Holland Province Netherlands:

• Research and development of virosome based vaccines.
• Utilized cell culturing, virus infection, TCID50, ultrafiltration and diafiltration, chromatography (ion-exchange, size exclusion), ELISA, report writing.
• In charge of the upstream process as well as involvement in the down process of vaccine preparation. Exercising GMP and SOP in BSL 2
conditions.



Barrett Stelze

3rd degree connection
3rd
Manager, Regulatory CMC at Sanofi Pasteur
Effort, Pennsylvania

Merck Biologist Viral Vaccine Technology & Engineering 2008: West Point, PA
Contract Position Through IT Solutions:

Responsibilities/Skills:

o Performed, optimized, and engaged in troubleshooting on: cell culture experiments, plaque assays, TCID50 potency assays, and Flow Cytometry assays, supporting viral vaccine processes (Rotavirus & MMR)
o Performed data analysis; generated spreadsheets; created, revised, and executed protocols and summary reports; and planned, scheduled, and prepared reagents & equipment for investigations
o Maintained and manipulated cell lines (Vero, ma104, wi38, rk13, and hct116) for process engineering studies

Matthijs Baart
Technician at Batavia Biosciences
Oegstgeest, South Holland Province, Netherlands

Within Mymetics I worked in a young and professional team that does the research for the company. The job consists of a variety of different techniques:

-ELISA
-TCID50
-Cell Culture
-Upstream / Downstream process
-Western Blotting
-Setting up and improving assays
-Nanosight




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