Jane Spirkoski
Molecular and Cell Biologist
The Hague Area, Netherlands
Mymetics Corp. Oct 2016 to Present. 2YRS 2MOS. Leiden, South Holland Province Netherlands:
• Research and development of virosome based vaccines.
• Utilized cell culturing, virus infection, TCID50, ultrafiltration and diafiltration, chromatography (ion-exchange, size exclusion), ELISA, report writing.
• In charge of the upstream process as well as involvement in the down process of vaccine preparation. Exercising GMP and SOP in BSL 2
conditions.
Barrett Stelze
3rd degree connection
3rd
Manager, Regulatory CMC at Sanofi Pasteur
Effort, Pennsylvania
Merck Biologist Viral Vaccine Technology & Engineering 2008: West Point, PA
Contract Position Through IT Solutions:
Responsibilities/Skills:
o Performed, optimized, and engaged in troubleshooting on: cell culture experiments, plaque assays, TCID50 potency assays, and Flow Cytometry assays, supporting viral vaccine processes (Rotavirus & MMR)
o Performed data analysis; generated spreadsheets; created, revised, and executed protocols and summary reports; and planned, scheduled, and prepared reagents & equipment for investigations
o Maintained and manipulated cell lines (Vero, ma104, wi38, rk13, and hct116) for process engineering studies
Matthijs Baart
Technician at Batavia Biosciences
Oegstgeest, South Holland Province, Netherlands
Within Mymetics I worked in a young and professional team that does the research for the company. The job consists of a variety of different techniques:
-ELISA
-TCID50
-Cell Culture
-Upstream / Downstream process
-Western Blotting
-Setting up and improving assays
-Nanosight