Kraig Biocraft Laboratories Inc (KBLB)

[first mike]

talking about accuracy of insertion location:

{mojo}“These problems were commonplace in early editing techniques such as piggyBac and zinc fingers. They are more rare with Crispr/CAS.“

The reality is a bit better than that.
Recombination techniques prior to PiggyBAC were virtually uncontroled with regard to location. The odds of success were literally millions to one.
PiggyBAC was a vast improvement. It lowered the odds of success to somewhere between 1% and 0.1%.
Sangamo's version of zinc fingers, CompoZr, is actually quite good at exact location placement. It uses a a key template 9 to 15 units long, where each unit is a sequence of 3 base pairs to match a chromosomal location.
ZFN CompoZr's greatest flaw is that it is expensive and time consuming to create the proper key.
Crispr-CAS has the advantage of being much cheaper and easier to use.
I am not certain of its exact location finding mechanism (Jazz ?) but I believe that it is as accurate as CompoZr or even a bit better.
CompoZr however is/was good enough. Sangamo achieved 99++ percent accuracy of placement to a single base-triple.
MS was created using PiggyBAC but may have been refined using CompoZr at a later date.
DS was created using CompoZr and perhaps refined or modified using Crispr
The current work at KBLB uses Crispr.

Mike L.