We have developed a simple and cost-effective technique for preparing representative, strand-specific RNA-Seq libraries. In this technique, called “Peregrine,” short non-identical tag sequences are incorporated at the 5' and 3' ends of the first strand of cDNA during its synthesis. These tags preserve strand specificity information, and are used as
Nothing novel, impressive or valuable about this. It is just another attempted distraction from the failure of PPHM management. It is predictable and sad.