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Sunday, February 26, 2017 2:16:00 PM
Improved methods of isolating and concentrating tumor exosomes were recently reported, which are based on the surprising use of acetate buffers to precipitate only PS-positive exosomes
In one embodiment, the assay may employ a protein microarray of PS binding agents. An array is generally defined as a surface onto which a variety of physically segregated reactants are deposited. The resulting discrete physical locations for the reactants may be referred to as "reaction sites." The surface may be a planar surface such as a glass, plastic or silicon slide, a microliter plate, a filter, a nitrocellulose or PVDF membrane or a bead to which a variety of capture proteins are bound. In the case of a glass, plastic or silicon slide or bead, the surface can be modified to create distinct reaction sites that bind the reactants and subsequently applied samples thereby preventing them from leaving the particular reaction site, i.e., to remain physically attached to the surface and segregated from one another. Protein immobilizing agents include various polymers, polyacrylamide gels, or surface modifications with amines, aldehydes or epoxy . The surface may also be a non-planar surface, such as a plate with multiple wells, where the wells serve the purpose of segregating reactants and samples. The major advantages of arrays are to permit highly sensitive automated, standardized and high throughput assay s. Another value of using an array is the ability to multiplex reactions.
This patent is tough reading for a wookie but boy oh boy the nuggets are great!
We don't need no stinking graphene...unless it's for nano scaffoldng.
Wooking AMAZING.
wook
Best of $RESULTS for Humanity, FDA Approval(s), Partner(s) Production, Imaging and MOST IMPORTANTLY!!!
Research.
Always looking deeper. RIP Dr. Thorpe
