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Re: aikifredicist post# 284598

Friday, 01/27/2017 9:56:45 AM

Friday, January 27, 2017 9:56:45 AM

Post# of 347009
from my brief research it appears they were looking for ovarian...

if that's the case.... many tests for others....

from abstract...

Exosomes from patient samples
5 µL of 4 µm aldehyde-activated latex beads (4%
w/v) (Invitrogen) were incubated with 1N11-T (12.5 µg)
at 4oC overnight. The beads were then washed, blocked
for 1 hr with 1% BSA and mixed with 300 µL of a 1/6
dilution of patient plasma for 2 hrs at 20oC. The beads
were then washed with HEPES-saline and a 1/100
dilution of mouse anti-human CD63 antibodies (Sigma
#SAB4700215) for 30 min. The beads were then washed
and stained for mouse Ig and PS with a 1/500 dilution of
FITC-goat anti-mouse Ig (Jackson Labs #115-095-166)
and a 1/100 dilution of Cy5-annexin 5 (Biovision #1013-
200) in HEPES-saline containing Ca2+ (1 mM). Samples
were screened and analyzed as described above.


https://en.wikipedia.org/wiki/CD63

A recent investigation showed that expression of CD63 positively correlates with the invasiveness of ovarian cancer.[4]
In cell biology, CD63 is often used as a marker for multivessicular bodies, which are enriched with CD63. [5]
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