Replies to post #24015 on NorthWest Biotherapeutics Inc (NWBO)

[flipper44]

Beachlifeisfun said:

Additionally, while not explicitly identified, the lysate process for -L is pretty brutal...it most assuredly destroys all of the tertiary and quaternary structure of the antigen(s), and likely some of the more fragile primary and secondary structure as well...Direct will allow phagocytosis in situ, meaning much of these structures will be preserved...again likely making Direct a more potent treatment.

This is something I thought about but quickly forgot to research. It may very well be that natural processing breaks down antigens at more appropriate junctions. I'll need to follow through this time.

[longusa]

Beach, very interesting comment. I had not thought about the lysate process and the possible damaging effect. Quite interesting. Can I ask if this is from experience you have in the biotech/biology space.

Re BP, the other reason they may stay away is that LP controls >50% of NW, so they have no leverage.

[Smokey21]

Also, the DC manufacturing process is different in DIRECT currently, in that it employs Tangential Flow Filtration (TFF), which according to the patent news release results in more potent DCs.

From the Sept 10, 2013 press release:

"Most significantly, the dendritic cells produced through this next generation system, are manufactured using procedures which are more closely related to what one finds in nature. The result is increased potency of the dendritic cells (which are the active agent in the DCVax products)."

My understanding it that the increase in potency is 10X, based on what I read somewhere else. This could also explain why DIRECT is showing such strong efficacy. Also, it would be a potential enhancement to the current L process at some point. I would guess they can't change the manufacturing process in the middle of the Phase III trial.

[maverick_1]

Beachlifeisfun: THKS for supplying "the elementS to the missing link" debunking the widely held view. Most of us? have sensed that Direct would be lower to mfg. And your specific hands on experience and commentary is most appreciative in the credence of your findings.

Now IF only the denizens or the skeptics in the industry get wind of this through other peer findings as well as NWBO data, along with numerous catalysts many have outlined.............. the upside begins. The process has been a labour of love/hate transitioning slowly so far for the masses at large towards the former.

AND most especially to Flipper for his "Tomb, in elaborating the differences for the masses to understand" that Beachlifeisfun is referencing.

And yes Longusa, "one of the impediments" for both Big Rx and institutions has been LP et al's control interest.