NorthWest Biotherapeutics Inc (NWBO)

[hyperopia]

skitahoe, I know for certain that Saint Gobain does not “load anything into the culture cartridge” when it’s manufactured. Saint Gobain manufactures lab supplies, not cell-therapy treatments.

In this case, the Eden cartridge is manufactured in a similar way as other tissue-culture flasks. The plastic polystyrene surface on the bottom of the Eden cartridge is subjected to a plasma gas treatment in order to make it more hydrophilic. The resulting surface carries a net negative charge which increases cell attachment. Additionally, the cartridges are likely sterilized using gamma irradiation to prevent contamination.

The patient’s monocytes are adherent cells, so in the lab, when the monocytes are loaded into the Eden cartridge, they adhere to the bottom surface. When the culture medium is perfused into the cartridge, the cells attached to the bottom are continually surrounded with culture medium.