Replies to post #142044 on Enzolytics Inc (ENZC)

[foxi]

This document from Rosetta mentions fetal bovine serum in EnzoImmune Active:

IPF was dissolved to an initial concentration of 50 mg/ml (background solution), of which ex tempore were prepared serial dilutions in a DMEM growth medium (Dulbecco's Modified Eagle Medium, Sigma-Aldrich, Germany), containing 2% fetal bovine serum (FBS, Gibco) and 1% antibiotic solution (100 U/ml penicillin and 100 µg/ml streptomycin sulphate (Gibco, USA) (working solutions), which were filtered through sterile antibacterial filters with a pore diameter of 0.45 µm (Sartorius Stedium, Australia)

https://enzoimmune.com/wp-content/uploads/2021/10/finalen-otchet-tzitotoksichen-efekt-na-ipf-eng.pdf

Rosetta quotes:

THE ACTIVE SUBSTANCE is Inactivated pepsin fragment (IPF) derived from pepsin from mucosa (CAS ? 9001-75-6) and the method of its extraction and application is patented in the USA as follows: US Patent ? 8,309,072 – Irreversible inactivated pepsinogen fragment for modulating immune function.

https://enzoimmune.com/

The patent quotes:

The following Examples are examples of methods for isolating, purifying, and characterizing the IPF compositions disclosed herein from active pig pepsinogen.

https://patents.google.com/patent/US8309072B2/en