Replies to post #320553 on Innovation Pharmaceuticals Inc (IPIX)

[Jhawker]

It says high si but what is the number?

[Here Today]

Line 42 on the chart probably a typo in article:


42 Remdesivir 0.77 >100 >129.87


https://ghddi-ailab.github.io/Targeting2019-nCoV/preclinical/

[KMBJN]

Different labs will get different SI with different methods (and random variability). They are reporting the remdesivir SI from this study:

https://www.sciencedirect.com/science/article/pii/S0166354220302928

IC50 (M) CC50 (M) SI
Remdesivir 0.987 275 278.62


Vero E6 cells were infected by SARS-CoV-2 at a MOI of 0.01 and treated 1 hpi


The StarPharma drug had different SI depending on the different methods used:

140 3.1 Virus-induced cytopathic effect inhibition assay

151 The selectivity index (SI) for astodrimer sodium against SARS-CoV-2 ranged from 793 to 2197
152 for the initial assays where compound was added 1 hour prior to infection and 1 hour after
153 infection, respectively, and was >70 to >80 in the repeat assays, in which cytotoxicity was not
154 observed up to the highest concentration tested (0.86 mg/mL).
155 The positive control, remdesivir, was also active in the CPE inhibition assay, with a SI of >33.

156 3.2 Virucidal assay

157 A second study was performed to determine if astodrimer sodium was able to reduce viral
158 infectivity by binding to SARS-CoV-2 directly prior to infection of Vero E6 cells. Astodrimer
159 sodium treatment demonstrated a similar level of antiviral efficacy to the CPE studies (Table 1;
160 Figure 1C) with an EC50 of 1.83 µM (0.030 mg/mL) and SI of 130; n=1.

These studies are all in Vero E6 cells, which don't predict as well as human lung cells.