Replies to post #81775 on Innovation Pharmaceuticals Inc (IPIX)

[TOB]

Brilliant research compilation Shinsekai, very much appreciated.

[tombrady12nh]

Amazing Post! Thanks

[Ultimate01]

Very thorough! impressive! Thank you for sharing the knowledge! I'm no where near reading everything bet felt that you should be thanked!

GO CTIX and GO P!!!

[rosalieone]

Wow Thank you !!

[TruongKy]

ShinSekai's post should be pinned as a sticky note

[I Need Help]

ShinSekai, an amazing post, chock full of information.

Funny how the market comes to value or not value some things (and information). I still feel CTIX is being underappreciated and undervalued on all fronts. B is starting to get some respect, but still underappreciated.

The pendulum swings.

[John B]

Great post, Shin. Thank you!

[Talc Moan]

Amazing Post Shin!

Thank You for taking the time to educate us all!

[scgmck1]

Great post Shin!!! Thanks for the superb work!!!

[Astavakra]

Shin, Thanks for the research. I'll wade through it deeper later. Would you have an idea of one or two example of the 'special amino acids' mentioned here?:

A large group of kinases are protein kinases, which catalyze the transfer to a special amino acid in most cases with a free hydroxyl group.

I'm not well informed on this topic. Thanks again.

[tombrady12nh]

ShinSekai-what is your occupation? I can't believe you put that entire post together by yourself in such a short amount of time.
Go CTIX

[WILD_4_IPIX]

UNBELIEVABLE ! Thanks Shin....I think you did just fine ! In fact I think I need to go get a bucket and clean up this mess as my brain just exploded : ) I LOVE this BOARD as there are so many unselfish and incredibly knowledgeable and considerate members whose contributions are compelling. Thanks again Pal !

GO CTIX !

[noretreat]

That is quite a summary! thanks for posting it.

[someconcerns]

Wow.

[Smooth]

Indeed an amazing post. I wish to heck it was written in (understandable) English. It is way over my head to understand, but I'm going to assume it means that P is "da bomb!"

Hey Wild4CTIX, when you get a minute, would your translate this paragraph for Shin's email PLEASE:

"It is possible that the context of the combined motifs has a profound effect on their function or that small variations in the sequencescan modulate the response(interferon inducible) or even abolish it, as is the case for protein binding for the example of the single base change shown in Figure 6B. While everything is consistent with a major role for the 14 nucleotide sequence, flanking sequences and additional factors may well play an important role in modulating the response."

[KMBJN]

Re: "I whish I had more time to put it altogether concisely for you guys." You and me and about a hundred other CTIX board readers!

Based on what you said in your most recent post and the previous one on the topic:

http://investorshub.advfn.com/boards/read_msg.aspx?message_id=107787928

"Sorry about that. I will post a summary of my findings soon. I was really excited and wanted to post that as soon as I found it. I'll just say our drug acts on PRINS and Il-6 related pathways similar to existing drugs just further downstream. The antiviral aspects of Abacavir don't really apply so don't get caught up with Carbovir's interactions with HIV. I hate to wet everyones' appetite and run. This is the best explanation of the pathway here ..."

It sounds like you keep saying that even though the Phase I study examined bioequivalence with abacavir sulfate (Ziagen), and we are applying under 505(b)2 as another form of abacavir, that the active ingredient in our drug against psoriasis is not in fact related to abacavir's nucleoside analog function (carbovir triphosphate, the metabolite with antiviral properties against viral reverse transcriptase - and why it is used for HIV), is that correct?

If so, can you please answer simply what is the active metabolite in prurisol (if it's not carbovir triphosphate), what is the mechanism of action, and what is the target pathway in psoriasis?

It "acts on PRINS and IL-6" which is what the company has stated before (well, PRINS and IL-20), but without saying how. Your latest post has amalgamated lots of interesting articles, but without any conclusion...

But if I had to guess, I think you are saying in general, it appears to act as an anti-metabolite like Lanvis and methotrexate, which will interfere with DNA synthesis and prevent skin cell hyperproliferation (psoriasis).

Abacavir is a nucleoside analog, which inhibits viral reverse transcriptase, but you appear to be saying our prurisol acts more as a nucleobase analog (ala Lanvis) or folic acid analog (methotrexate), which both seem to inhibit DNA synthesis, but also might have immunosuppressant and anti-inflammatory properties, correct?

Then again, maybe people will never understand how prurisol might work. After all, methotrexate was approved for psoriasis in 1971, and they still aren't sure how it works!

"The exact mechanism of action has not been fully elab-
orated but is theorised to act as an immunosuppres-
sant by inhibiting the inflammatory response rather
than acting as an antiproliferative agent as pre-
viously thought."

http://www.medscape.com/viewarticle/503441_5

Thanks for taking the time to try to help us all understand the science behind prurisol.

[ShinSekai]

Why Tris-acetate?
en.wikipedia.org/wiki/Tris
http://www.sciencedirect.com/science/article/pii/0049384886902665
http://books.google.com/books?id=nMm2WfrH3fIC&pg=PR5&lpg=PR5&dq=nucleosides+sulfate+acetate&source=bl&ots=GPq4DNJ8Og&sig=pv_h5VvVIdy28Uq72GyTwkVkZ3s&hl=en&sa=X&ei=sItdVJCjL9CxogTLr4AI&ved=0CC4Q6AEwAw#v=onepage&q=nucleosides%20sulfate%20acetate&f=false

It should be *Hydroxymethyl Acetate



Why Hydroxymethyl acetate?
http://en.wikipedia.org/wiki/Hydroxymethyl
http://www.nature.com/jid/journal/v132/n3-1/full/jid2011364a.html
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262011/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568942/

Diagram shows the split into two moieties

http://books.google.com/books?id=nMm2WfrH3fIC&pg=PR5&lpg=PR5&dq=nucleosides+sulfate+acetate&source=bl&ots=GPq4DNJ8Og&sig=pv_h5VvVIdy28Uq72GyTwkVkZ3s&hl=en&sa=X&ei=sItdVJCjL9CxogTLr4AI&ved=0CC4Q6AEwAw#v=onepage&q=nucleosides%20sulfate%20acetate&f=false