Genentec/Roche is interested in BavImag. The Koji paper which follows, from Japan, in a clinical sense is a curiosity, almost amusing, when compared in applicability to BavImag. Wonder what MAB, if any they are using.
In Vivo Time-Course Imaging of Tumor Angiogenesis in Colorectal Liver Metastases in the Same Living Mice Using Two-Photon Laser Scanning Microscopy
Koji Tanaka,1 Yuhki Morimoto,1 Yuji Toiyama,1 Kohei Matsushita,1 Mikio Kawamura,1 Yuhki Koike,1 Yoshinaga Okugawa,1 Yasuhiro Inoue,1 Keiichi Uchida,1 Toshimitsu Araki,1 Akira Mizoguchi,2 and Masato Kusunoki1
In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major challenge in metastasis research. We developed a technique for intravital imaging of colorectal liver metastasis development in live mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green). In vivo time-course imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug evaluation, reducing the effects of interindividual variation.
