Wednesday, January 13, 2010 3:05:26 AM
Mayo Clinic Report
Prostatic Intraepithelial Neoplasia Analysis
Immunohistochemistry for PCNA (Zymed, 93-1143) and Pten (Millipore,04-035) were performed according to instructions provided by the manufacturers.
SSLP analysis
For SSLP analysis, DNA extracted from normal and tumor samples of thymic lymphoma bearing Bub1–/H/p53+/– mice were genotyped using fluorescently labeled PCR primers to amplify six SSLP markers located along chromosome 11 (Applied Biosystems). PCR reactions were performed according to manufacturer’s instructions using TrueAllele PCR premix (Applied Biosystems). In order to determine the sizes of the PCR product produced, 2 µl of this reaction was added to 10 µl of Hi-Di formamide plus LIZ (minus 250) internal lane standards and run on an ABI 3730XL DNA Analyzer (Applied Biosystems). Raw data was analyzed using GeneMapper software (Applied Biosystems).
Supplemental Report
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Millipore
Anti-PTEN, clone 6H2.1
Catalog
Applied Biosystems
The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing.
3730XL DNA Analyzer
GeneMapper
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BioDot Inc.
Affiliates:
BioTrove Inc.
Millipore
Affilitates | Consumable Suppliers
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Life Technologies Completes Acquisition of BioTrove
December 15, 2009 - Life Technologies Corporation (NASDAQ:LIFE), a provider of innovative life science solutions, today announced that it has completed the previously announced acquisition of BioTrove, Inc. for an undisclosed amount.
BioTrove's primary technology is the OpenArray(R) platform, a high throughput gene expression and genotyping analysis system based on a flexible array format that enables researchers to perform more than 3,000 PCR genotyping or qPCR gene expression assays at a time.
BioTrove News
Life Technologies' Brands
Applied Biosystems
Applied Biosystems is a global leader in providing innovative instrument systems to accelerate academic and clinical research, drug discovery and development, pathogen detection, environmental analysis, and forensic DNA analysis.
Life Technologies
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