NorthWest Biotherapeutics Inc (NWBO)

[eagle8]

d_stock
@d_stock07734
Size exclusion chromatography (SEC) is the analytical “gold standard” for the separation and accurate quantitation of aggregates, monomers, and lower molecular weight fragments contained in biotherapeutic peptides and proteins, including monoclonal antibody (mAbs) drugs.

Did Dr. Liau use SEC in her research? Sure did.
https://ncbi.nlm.nih.gov/pmc/articles/PMC2818760/pdf/nihms165905.pdf

"For enzyme assays using recombinant IDH1 enzyme, proteins were purified from E coli using Ni affinity and size-exclusion chromatography."

The interesting part is that all these BPs are recruiting people with skills on SEC.

Merck
Principal Scientist, Biologics Analytical Research & Development
https://jobs.merck.com/us/en/job/R281389/Principal-Scientist-Biologics-Analytical-Research-Development

Extensive experience in analysis of biologics (separation methods such as Size Exclusion Chromatography (SEC), Ion Exchange Chromatography (IEX) and Capillary Electrophoresis (CE) techniques, and immunoassays such as residual and PCR-based assays).

AMGEN
Process Development Associate
https://biospace.com/job/2874322/process-development-associate
Performing analytical testing in the realm of chromatography and electrophoresis involves various techniques to separate, identify, and quantify components within a sample most including the techniques below:
Size Exclusion Chromatography (SEC)
Reduced Capillary Electrophoresis (rCE-SDS)
Non-Reduced Capillary Electrophoresis (nrCE-SDS)

Astrazeneca
Senior Scientist, Bioconjugation
https://careers.astrazeneca.com/job/gaithersburg/senior-scientist-bioconjugation/7684/61310462272
Proficiency in analytical characterization techniques such as Hydrophobic Interaction Chromatography (HIC)-HPLC, Size Exclusion Chromatography (SEC), Liquid Chromatography–Mass Spectrometry (LC-MS), Ion-Exchange (IEX), and Reversed Phase (RP)-HPLC.

Takeda
Senior Research Associate
https://glassdoor.com/job-listing/senior-research-associate-takeda-pharmaceuticals-JV_IC1154599_KO0,25_KE26,48.htm?jl=1008450619804

Analyze protein aggregation by Size Exclusion chromatography (SEC)

Bristol Myers Squibb
Scientist Protein Expression and Purification
https://linkedin.com/jobs/view/scientist-protein-expression-and-purification-at-bristol-myers-squibb-3834016179/

Perform mini to mid-scale purifications using different kinds of chromatography (affinity, ion exchange, size exclusion and hydrophobic interaction chromatography) and be responsible for purification process design, optimization and troubleshooting if needed

Regeneron
Sr. Assoc. Scientist Protein Biochemistry
https://glassdoor.com/job-listing/sr-assoc-scientist-protein-biochemistry-regeneron-pharmaceuticals-inc-JV_IC1132438_KO0,39_KE40,69.htm?jl=1009076902423

Fundamental technical competencies in key analytical protein methodologies such as Size Exclusion Chromatography (SEC), UV-Vis spectrophotometry, SDS-PAGE, Western blotting, CE-SDS, protein purification and basic chemistry skills as applied toward protein modifications.

Abbvie
2024 Biologics Drug Product Development Intern
https://biospace.com/job/2883970/2024-biologics-drug-product-development-intern/?utm_campaign=google_jobs_apply&utm_source=google_jobs_apply&utm_medium=organic
Identify relative risk of protein adsorption and degradation under various formulation conditions using analytical techniques, such as: quartz crystal microbalance with dissipation (QCM-D); size exclusion chromatography (SEC); particle characterization (micro-flow imaging); and ion-exchange chromatography (IEX)

Eli lilly
Advisor / Sr. Advisor - Separation Science and Mass Spectrometry - Bioproduct Research and Development
https://careers.lilly.com/us/en/job/R-62167/Advisor-Sr-Advisor-Separation-Science-and-Mass-Spectrometry-Bioproduct-Research-and-Development?utm_campaign=google_jobs_apply&utm_source=google_jobs_apply&utm_medium=organic
Experience with chromatographic separations (reversed phase chromatography, ion exchange chromatography, size exclusion chromatography, affinity chromatography), capillary electrophoresis, and other methods for the analysis of oligonucleotides, peptides, and other biomolecules.

J&J
SENIOR SCIENTIST PROTEIN SCIENCES
https://johnsonjohnson.ongig.com/jobs/r-d/-san-diego-california-united-states/senior-scientist-protein-sciences/6830190611#/

Proficiency in use of LCMS, liquid chromatography (including HPLC, affinity purification, size exclusion chromatography, ion exchange chromatography), and associated data analysis is required.