EDUCATE YOURSELF
Let me explain how an experiment work.
COMMON SENSE
Every Biopharma since very long long time ago, after they medicated a human with certain medicine and that human died, many years ago, the Entity in charge of the pharmaceutical approvals decided to use rats for experiments? Why? Because the life of a human being is consider more valuable.
LETS START!!!
So how i know a medicine works in a rat???
Thru induction ( this is just one of several methods). When you induce some kind of chemicals into an animal which will cause the condition you are targeting, then the game is on.
Once you prove that the rat is infected, then you can try your method to see if it does works or not. For example, let see some extract from the TSOI US Patent for Nutraceuticals for suppressing indolamine 2,3 deoxygenase Patent (Patent # 11,229,674)
Type: Grant
Filed: Oct 23, 2020
Date of Patent: Jan 25, 2022
Assignee: Therapeutic Solutions International, Inc. (Oceanside, CA)
Inventors: Thomas E. Ichim (Oceanside, CA), Timothy G. Dixon (Oceanside, CA), James Veltmeyer (Oceanside, CA)
Primary Examiner: Aaron J Kosar
Application Number: 16/901,028
EXAMPLES ( this is how the experiment was conducted)
Example 1: QUADRAMUNE™ Preserves Memory in Inflammation Associated Memory Decline Model
Female BALB/c mice were treated with control, daily LPS treatment, and some with LPS and QUADRAMUNE™.QUADRAMUNE™ was administered daily by gavage at a concentration 1 (100 ug of broccoli sprout extract, Nigella sativa, and green tea extract, and 50 ug of pterostilbene), and concentration 2 (200 ug of broccoli sprout extract, Nigella sativa, and green tea extract, and 100 ug of pterostilbene).
To assess memory function, water filled basin which was 120 cm in diameter was broken into 4 quadrants. 10 cm diameter platform placed 1 cm below water. Mice were forced to swim to find the hidden platform, starting from all four different quadrants, each day for 7 days. The time was recorded if they could find the hidden platform in 60 s . . . . If not, mice are guided toward the platform and allowed to stand on it for 10 s. Results are shown in FIG. 1.
Example 2: QUADRAMUNE™ Reduces Inflammation Associated Kynurenine Elevation
Blood samples were collected from mice in Example 1 and assessed for Kynurenine content. Augmented levels of kynurenine were observed in LPS treated mice as compared to controls. Results are shown in FIG. 2.
QUADRAMUNE HAD BEEN SCIENTIFICALLY PROVEN TO HAVE GREAT RESULTS. THIS ARE THE CLAIMS
:
Claims
1. A method of inhibiting indolamine 2,3 deoxygenase (IDO) expression or activity, comprising: administration to a patient in need a therapeutic combination comprising: a) Green Tea and/or extract thereof; b) Blueberry and/or extract thereof; c) Nigella sativa and/or extract thereof; and d) broccoli and/or extract thereof.
2. The method of claim 1, wherein said green tea extract is epigallocatechin-3-gallate or an analogue thereof.
3. The method of claim 1, wherein said blueberry extract is pterostilbene or an analogue thereof.
4. The method of claim 1, wherein said Nigella sativa extract is thymoquinone or an analogue thereof.
5. The method of claim 1, wherein said broccoli extract is sulforaphane or an analogue thereof.
6. The method of claim 1, wherein inhibition of IDO expression and/or activity in the host is associated with enhancement of natural killer cell activity.
7. The method of claim 6, wherein said natural killer cell activity is quantified by ability to lyse a virally infected cell.
8. The method of claim 6, wherein said natural killer cell activity is quantified by ability to lyse K562 cells.
9. The method of claim 6, wherein said natural killer cell activity is quantified by ability to lyse YAC-1 cells.
10. The method of claim 1, wherein inhibition of IDO expression and/or activity in the host is associated with enhancement of interferon production.
11. The method of claim 1, wherein inhibition of IDO expression and/or activity in the host is accomplished by enhancement of T cell activation.
12. The method of claim 11, wherein said T cell activation is induction of T helper cell 1 activity.
13. The method of claim 12, wherein said T helper cell 1 activity comprises production of interferon gamma.
14. The method of claim 11, wherein said T cell activation is induction of T cytotoxic cell activity.
15. The method of claim 1, wherein said patient is suffering from a tumor, and said therapeutic combination is administered at a dosage and frequency sufficient to suppress growth of a tumor.
16. The method of claim 15, wherein said tumor growth in the host is associated with suppression of cancer angiogenesis.
17. The method of claim 1, wherein said patient is suffering from COVID 19.
18. The method of claim 1, wherein the patient is suffering from memory loss.
19. The method of claim 1, wherein the patient has elevated levels of Kynurenine
AND I INVITE YOU TO THE JUSTIA SITE TO FIND HOW THE EXPERIMENTS WERE CONDUCTED SUCCESSFULLY FOR EVERY PATENT.
DO YOU SEE HOW TSOI IS WORKING TO IMPROVE THE LIFE QUALITY OF MILLIONS OF PEOPLE THAT COULD BE OUR RELATIVES?
Please do your own DD.
