NorthWest Biotherapeutics Inc (NWBO)

[hyperopia]

Thanks flipper, I don’t recall that a filter was part of the (MicroDEN) device, (and that seems like a detail that I would remember) but it has been a couple of years since I looked at the patent, and the Northeastern University paper. As I recall, step one was seeding the (already isolated) monocytes into the two culture chambers, where they are perfused with differentiation media. I know for DCVax-Direct, it’s all enclosed in one device, so I have thought that in the development of the Flaskworks’ commercial system, TFF would be incorporated eventually.

You make a very good point though. It’s probably better to look at the patents. I have thought of the two Flaskworks’ systems by the commercial names MicroDEN and BATON, and separated the technologies of each. The filter you describe may be a part of the BATON - I haven’t taken a deep dive on that system. After one of our previous exchanges, you got me thinking about the possibilities of combining the different elements of each system into a commercial system for each DCVax product. I hope that the brilliant Shashi Murthy and the technical staff that came with the Flaskworks acquisition, and Dr. Bosch, are way ahead of us on that.

Wasn’t it Donald Rumsfeld who said “you go to market with the manufacturing system that you have, not the one that you want,” or something like that. One of the questions that I’ve had is: how many resources have been directed toward the development work for this first commercial system? Are they still small, benchtop machines that must be put in incubators to control temperature and CO2 levels, or has this been enclosed in a larger cabinet where environment conditions can be controlled? Does it simply have in-process sensors and need to be adjusted by a technician, or has controlling that process also been automated? Are there multiple culture chambers or only one? Has TFF been incorporated? (I could go on)

I submitted a number of questions about Flaskworks for the annual shareholder meeting last year, but none were answered. I’m sure we’ll get some answers at the next one though.