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Wednesday, 02/21/2018 2:50:27 PM

Wednesday, February 21, 2018 2:50:27 PM

Post# of 2804248
$RGBP Determining the X-ray crystal structure of a protein and its ligand is a multi-step process:

High levels of the protein are expressed.
A saturated solution of protein is created in the presence of ligand. If the protein of interest has never been structurally determined before (as in the case of NR2F6), scores of different conditions must be tested. High levels of protein are needed in the first step, because ample protein is required to saturate a solution and to test many different conditions.
Finally, if crystals form, their structural features are determined. This is done by exposing the crystals to a very high-power x-ray beam. The x-ray beam hits the protein and diffracts. The pattern of diffraction is used to determine the structure. https://finance.yahoo.com/news/regen-biopharma-inc-program-determine-130000690.html
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