NorthWest Biotherapeutics Inc (NWBO)

[Doktornolittle]

Mostly wondering what they call "mesenchymal" if there is no resting state mesenchymal. Anything that is generating mesenchymal runners? If so, there would be multiple types of mesenchymal and you would expect efficacy to vary. DCVax-L might work on the runners, but what of the stationary tumor? It has to work there too.

.. unless as I was getting at, the resting tumor is actually in a losing battle already if not for the mesenchymal runners.

I did sort of understand what Doc was saying, but you have clarified it further. The underlying genetic code does not fully specify which antigens will be presented because it does not fully determine which proteins will be built. That gets modulated as per cell type / as the shape changer changes form. Thus the various implications that you two brought up.

Interesting... to me anyway, and probably to you (if not to a lot of frustrated investors not interested in this stuff)... the transition to mesenchymal can be spurred by stress... as you have said with the locust analogy, etc, and as it says in the papers you have quoted, including hypoxia. Removing the tumor is certainly stress... so if they wanted to generate more mesenchymal characteristic antigen, maybe they could put the tumor in luke warm saline for a little while instead of freezing it right away, allowing some transition to mesenchymal... or not. I wonder how long it takes... ie how long the tumor cells would have to be kept alive but hurting, for such transition to occur?

They do get to sterilize the tumor lysate later, I would suppose, as far as pathogen reproduction side effect goes. Freeze thaw may not be enough to kill pathogens... but if they really do a lysate procedure involving an alkaline agent then that would probably kill everything.