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es1

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es1

Re: FACT-MASTER post# 79625

Saturday, 08/30/2014 6:07:08 PM

Saturday, August 30, 2014 6:07:08 PM

Post# of 276233
What is this? About the 7th or 8th group to try the Amsilk method?
EXERPT #1

The cap­stone stu­dents col­lab­o­rated with Tufts Uni­ver­sity pro­fessor and spider silk expert David Kaplan to engi­neer a the­o­ret­ical method for pro­ducing the silk in bac­te­rial cells at cur­rently unmatched con­cen­tra­tions. Pre­vi­ously, researchers have man­aged to force bac­teria like Escherichia coli to pro­duce silk, but only in small amounts.

The silk gene sequence is large and highly repet­i­tive and cells have trouble repli­cating pro­tein from it, said Jones, who focused pri­marily on the bio­log­ical side of the project. So the young researchers decided to build their model using a genet­i­cally mod­i­fied E. coli strain that more effec­tively deals with the repet­i­tive silk protein.



EXERPT #2

Chem­ical engi­neering stu­dents Robert Jones, Thomas Khoury, and Richard Salvucci spent the last semester of their North­eastern under­grad­uate careers devel­oping a set of solu­tions that could make C. dar­wini silk fibers a viable option for industrial-??scale man­u­fac­turing.


EXERPT #3[

They incor­po­rated other muta­tions into their hypo­thet­ical E. coli strain to increase its effi­ciency and pro­tein pro­duc­tivity.



Not only do they not have the silk, they don't have the ecoli to produce it, and they don't have the machine to spin it. All they really have is a set of hypothetical solutions.
THAT IS A FACT

In about 6-8 years they should have a stable ecoli to work with. Let me know when they get there and we can talk about how they will get the gene into the ecoli. After that we can discuss the apparatus foe spinning.
These college kids should hook up with the college kid running Spiber and compare notes. Maybe when they are Kims age they may actually have something.

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