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Re: saintcalvus post# 73579

Thursday, 04/24/2014 1:26:33 PM

Thursday, April 24, 2014 1:26:33 PM

Post# of 276194

BR may be defective from the getgo.



Per the Wikipedia article you linked to:
"In the early 2000s, researchers developed zinc finger nucleases, synthetic proteins whose DNA-binding domains enable them to cut DNA at specific spots. Later, synthetic nucleases called TALENs provided an easier way to target specific DNA and were predicted to surpass zinc fingers. They both depend on making custom proteins for each DNA target, a more cumbersome procedure than guide RNAs. CRISPRs are more efficient and can target more genes than these earlier techniques."

This does not mean that BR would be defective, it just shows that CRISPR is a more advanced gene sequencing technique. It would be advantageous for Kim to look into this technology as it become available to further improve on the genetic variance of the silkworms, but it does not mean that the use of Zinc Fingers is defective.
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