You would wire the money directly from your bank/finanical institution. We're NOT talking Western Union type wire.
Check on the Ameritrade website to see when your funds got in...its usually 3 business days (today wont count nor do weekends) until it clears.
They will also have all the information you need to do a wire transfer from your bank or other financial institution. Realize these usually charge a fee of $25-$50.
When I have wired money from my bank at 0730 CST it has usually been available to trade by 1100 CST.