Replies to post #10079 on BioCurex, Inc.(fka BOCX)

[i_invest_utrade]

From the link:

Example 3

Autoantibody Bead Array

[0267] A. Commercially available human proteins (See, Table 4, below) were attached to Luminex.TM. SeroMap.TM. beads (Austin, Tex.) and the individual beadsets were combined to prepare the reagent. Portions of the reagent were exposed to the human serum samples under conditions that allow any antibodies present to bind to the proteins. The unbound material was washed off and the beads were then exposed to a fluorescent conjugate of R-phycoerythrin linked to an antibody that specifically binds to human IgG. After washing, the beads were passed through a Luminex.TM. 100 instrument, which identified each bead according to its internal dyes, and measured the fluorescence bound to the bead, corresponding to the quantity of antibody bound to the bead. In this way, the immune responses of 772 samples (251 lung cancer, 244 normal, 277 benign) against 21 human proteins, as well as several non-human proteins for controls (bovine serum albumin (BSA) and tetanus toxin), were assessed.

[0268] The antigens MUC-1 (Fujirebio Diagnostics INC, Malvern, Pa.), Cytokeratin 19 (Biodesign, Saco, Me.), and CA-125 (Biodesign, Saco, Me.) were obtained as ion-exchange fractions of cell cultures (See Table 4, below). These relatively crude preparations were subjected to further fractionation by molecular weight using HPLC with a size exclusion column (BioRad SEC-250, Hercules, Calif.) with mobile phase=PBS at 4 mL/minute. Fractions were collected starting at 15 minutes with 1 minute for each fraction for a total of 23 fractions for each antigen. For MUC-1, 250 uL was injected; for Cytokeratin 19 and CA-125, 150 uL was injected. All three samples showed signals indicating various concentrations of higher MW proteins eluting from 15-24 minutes, with signals too high to measure at times longer than 24 minutes, indicating high concentrations of lower MW materials. For coating on beads the following fractions were combined: MUC-1-A fractions 6, 7; MUC-1-B fractions 10, 11; MUC-1-C fractions Cytokeratin 19-A fractions 4, 5; Cytokeratin 19-B fractions 8, 9; Cytokeratin 19-C s 16, 17; CA125-A fractions 5, 6; CA125-B fractions 12, 13. TABLE-US-00005 TABLE 4 List of proteins. Bead ID Antigen Source 1 MUC-1-A Fujirebio Diagnostics INC 2 MUC-1-B Fujirebio Diagnostics INC 3 MUC-1-C Fujirebio Diagnostics INC 4 Cytokeratin 19-A Biodesign, Saco, ME 5 Cytokeratin 19-B Biodesign, Saco, ME 6 Cytokeratin 19-C Biodesign, Saco, ME 7 CA125-A Biodesign, Saco, ME 8 CA125-B Biodesign, Saco, ME 9 HSP27 US Biological, Swampscott, MA 10 HSP70 Alexis, San Diego, CA 11 HSP90 Alexis, San Diego, CA 12 Tetanus Sigma, St. Louis, MO 13 HCG Diosynth API, Des Plaines, IL 14 VEGF Biodesign, Saco, ME 15 CEA Biodesign, Saco, ME 16 NY-ESO-1 NeoMarkers, Fremont, CA 17 AFP Cell Sciences, Canton, MA 18 ERB-B2 Invitrogen, Grand Island, NY 19 PSA Fitzgerald, Concord, MA 20 P53 Lab Vision, Fremont, CA 21 JO-1 Biodesign, Saco, ME 22 Lactoferrin Sigma, St. Louis, MO 23 HDJ1 Alexis, San Diego, CA 24 Keratin Sigma, St. Louis, MO 25 RECAF62 BioCurex, Vancouver, BC Canada 26 RECAF50 BioCurex, Vancouver, BC Canada 27 RECAF milk BioCurex, Vancouver, BC Canada 28 BSA Sigma, St. Louis, MO

[mcd2inga]

That's great! I scanned it too fast then. I will look again.
Thanks!