Replies to post #270481 on Kraig Biocraft Laboratories Inc (KBLB)

[EOT]

Your understanding on everything about Kraig Labs is juvenile! Kraig has one of the BEST patent/IP legal firm’s in the country.

When you submit your invention to the patent office it is protected. When Pending it can be elaborated upon or simplified. And extended for quite a long time in this state (intentional??), but when the patent is granted. The 20-30year protection is backdated to the first day it was filed.



Quote:
1.WO/2022/016053-SYNTHESIS OF NON-NATIVE PROTEINS IN BOMBYX MORI BY MODIFYING SERICIN EXPRESSION
WO - 20.01.2022
Int.Class
A01K 67/04
Appl.No PCT/US2021/041968
Applicant KRAIG BIOCRAFT LABORATORIES, INC.
Inventor THOMPSON, Kimberly Kraig
Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with sericin proteins. Embodiments include vectors utilized for modifying one or more sericin genes. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into a targeted sericin gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing a non-native protein product with minimized or prevented production of sericin.

2.WO/2022/016052-SYNTHESIS OF HIGH MOLECULAR WEIGHT PROTEINS USING INTEINS
WO - 20.01.2022
Int.Class
A01K 67/04
Appl.No PCT/US2021/041965
Applicant KRAIG BIOCRAFT LABORATORIES, INC.
Inventor THOMPSON, Kimberly Kraig
This disclosure is directed to split intein protein production systems using transgenic target organisms such as Bombyx mori. A vector set for transforming a target organism includes: a first vector having a first donor sequence that encodes (i) a first non-native protein and (ii) at least one split intein domain; a second vector having a second donor sequence that encodes (i) a second non-native protein and (ii) at least one split intein domain. The respective split intein domains encoded by the first and second vectors are configured to associate with one another and ligate the first and second non-native proteins to thereby form a fused protein.

3.20220017918-SYNTHESIS OF NON-NATIVE PROTEINS IN BOMBYX MORI BY MODIFYING SERICIN EXPRESSION
US - 20.01.2022
Int.Class
C12N 15/85
Appl.No 17377318
Applicant Kraig Biocraft Laboratories, Inc.
Inventor Kimberly Kraig Thompson
Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with sericin proteins. Embodiments include vectors utilized for modifying one or more sericin genes. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into a targeted sericin gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing a non-native protein product with minimized or prevented production of sericin.

4.20220017917-SYNTHESIS OF HIGH MOLECULAR WEIGHT PROTEINS USING INTEINS
US - 20.01.2022
Int.Class
C12N 15/85
Appl.No 17377312
Applicant Kraig Biocraft Laboratories, Inc.
Inventor Kimberly Kraig Thompson
This disclosure is directed to split intein protein production systems using transgenic target organisms such as Bombyx mori. A vector set for transforming a target organism includes: a first vector having a first donor sequence that encodes (i) a first non-native protein and (ii) at least one split intein domain; a second vector having a second donor sequence that encodes (i) a second non-native protein and (ii) at least one split intein domain. The respective split intein domains encoded by the first and second vectors are configured to associate with one another and ligate the first and second non-native proteins to thereby form a fused protein.

5.20190185528-TRANSGENIC SILKWORMS CAPABLE OF PRODUCING CHIMERIC SPIDER SILK POLYPEPTIDES AND FIBERS
US - 20.06.2019
Int.Class
C07K 14/435
Appl.No 16275159
Applicant University of Notre Dame du Lac
Inventor Malcolm James Fraser
Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

6.20150322121-CHIMERIC SPIDER SILK POLYPEPTIDES AND FIBERS AND USES THEREOF
US - 12.11.2015
Int.Class
C07K 14/435
Appl.No 14754916
Applicant THE UNIVERSITY OF NOTRE DAME
Inventor Malcolm James FRASER
Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

7.20190153047-TRANSGENIC SILKWORMS CAPABLE OF PRODUCING CHIMERIC SPIDER SILK POLYPEPTIDES AND FIBERS
US - 23.05.2019
Int.Class
C07K 14/435
Appl.No 16246318
Applicant University of Notre Dame du Lac
Inventor Malcolm James Fraser, Jr.
Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

8.20210246471-MODIFICATION OF HEAVY CHAIN FIBROIN IN BOMBYX MORI
US - 12.08.2021
Int.Class
C12N 15/90
Appl.No 17172818
Applicant Kraig Biocraft Laboratories, Inc.
Inventor Trevor Logan Kane
Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with the heavy chain fibroin protein. Embodiments include insertion and truncation vectors utilized for modifying the FibH gene. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into the FibH gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing an enhanced silk product with a high percentage of spider silk proteins. Embodiments include a silk product produced by such transgenic Bombyx mori.

9.WO/2021/163249-MODIFICATION OF HEAVY CHAIN FIBROIN IN BOMBYX MORI
WO - 19.08.2021
Int.Class
C12N 15/90
Appl.No PCT/US2021/017544
Applicant KRAIG BIOCRAFT LABORATORIES, INC.
Inventor KANE, Trevor Logan
Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with the heavy chain fibroin protein. Embodiments include insertion and truncation vectors utilized for modifying the FibH gene. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into the FibH gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing an enhanced silk product with a high percentage of spider silk proteins. Embodiments include a silk product produced by such transgenic Bombyx mori.

10.20150322122-TRANSGENIC SILKWORMS CAPABLE OF PRODUCING CHIMERIC SPIDER SILK POLYPEPTIDES AND FIBERS
US - 12.11.2015
Int.Class
C07K 14/435
Appl.No 14754946
Applicant THE UNIVERSITY OF NOTRE DAME
Inventor Malcolm James FRASER
Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.



Table of Patent Applications and Status from 10k

Title Country Application No. Filing Date Patent No. Patent Date Status
Chimeric Spider Silk Polypeptides and Fibers Uses Thereof United States of America 14/754916 30-Jun-2015 Under Exam


Transgenic Silkworms Capable of Producing Chimeric Spider Silk Polypeptides and Fibers United States of America 14/754946 30-Jun-2015 Under Exam


A Nucleic Acid Encoding a Chimeric Spider Silk Polypeptide, Chimeric Spider Silk Polypeptide, Composite Fiber Comprising the Chimeric Spider Silk Polypeptide and Method of Preparing a Transgenic Silkworm Vietnam 1-2013-01306 25-Apr-2013 26612 03-Nov-2020 Granted


Method of Preparing a Transgenic Silkworm, Transgenic Silkworm, Manufacturing Method for the Production of Chimeric Spider Silk Composite Fiber and Genetic Construct Vietnam 1-2020-05354 17-Sep-2020 Pending


Chimeric Spider Silk and Uses thereof Australia 2011314072 26-Apr-2013 2011314072 13-Jul-2017 Granted


Chimeric Spider Silk and Uses thereof Canada 2812791 28-Sep-2011 2812791 14-Jul-2020 Granted


Chimeric Spider Silk and Uses thereof China (People’s Republic) 201180057127.1 28-May-2013 Pending


Chimeric Spider Silk and Uses thereof European Patent Convention 11833071.1 26-Apr-2013 262195702-Jun-2021 EP Granted


Chimeric Spider Silk and Uses thereof Germany 11833071.1 26-Apr-2013 602011071095.8 02-Jun-2021 Granted


Chimeric Spider Silk and Uses thereof France 11833071.1 26-Apr-2013 2621957 02-Jun-2021 Granted


Chimeric Spider Silk and Uses thereof India 3574/DELNP/2013 22-Apr-2013 Under Exam


Chimeric Spider Silk and Uses thereof Japan 2013-530432 26-Mar-2013 Pending


Chimeric Spider Silk and Uses Thereof Japan 2017-038829 01-Mar-2017 Under Exam


Chimeric Spider Silk and Uses thereof Korea, Republic of 10-2017-7005086 22-Feb-2017 10-1926286 30-Nov-2018 Granted


Chimeric Spider Silk and Uses thereof Korea, Republic of 10-2018-7034773 30-Nov-2018 10-2063002 30-Dec-2019 Granted


Modification of Heavy Chain Fibroin In Bombyx Mori United States of America 17/172818 10-Feb-2021 Published


Modification of Heavy Chain Fibroin In Bombyx Mori Patent Cooperation Treaty PCT/US2021/017544 11-Feb-2021 Published


Modification of Heavy Chain Fibroin In Bombyx Mori European Patent Convention 21753525.1 07-Sep-2022 Published


Synthesis of High Molecular Weight Proteins Using Inteins United States of America 17/377312 15-Jul-2021 Published


Synthesis of High Molecular Weight Proteins Using Inteins Patent Cooperation Treaty PCT/US2021/041965 16-Jul-2021 Published


Synthesis of High Molecular Weight Proteins Using Inteins European Patent Convention 21841571.9 20-Jan-2023 Pending


Synthesis Of Non-Native Proteins In Bombyx Mori By Modifying Sericin Expression United States of America 17/377318 15-Jul-2021 Published


Synthesis Of Non-Native Proteins In Bombyx Mori By Modifying Sericin Expression Patent Cooperation Treaty PCT/US2021/041968 16-Jul-2021 Published


Synthesis Of Non-Native Proteins In Bombyx Mori By Modifying Sericin Expression European Patent Convention 21843411.6
Pending