1.WO/2022/016053-SYNTHESIS OF NON-NATIVE PROTEINS IN BOMBYX MORI BY MODIFYING SERICIN EXPRESSION WO - 20.01.2022 Int.Class A01K 67/04 Appl.No PCT/US2021/041968 Applicant KRAIG BIOCRAFT LABORATORIES, INC. Inventor THOMPSON, Kimberly Kraig Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with sericin proteins. Embodiments include vectors utilized for modifying one or more sericin genes. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into a targeted sericin gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing a non-native protein product with minimized or prevented production of sericin.
2.WO/2022/016052-SYNTHESIS OF HIGH MOLECULAR WEIGHT PROTEINS USING INTEINS WO - 20.01.2022 Int.Class A01K 67/04 Appl.No PCT/US2021/041965 Applicant KRAIG BIOCRAFT LABORATORIES, INC. Inventor THOMPSON, Kimberly Kraig This disclosure is directed to split intein protein production systems using transgenic target organisms such as Bombyx mori. A vector set for transforming a target organism includes: a first vector having a first donor sequence that encodes (i) a first non-native protein and (ii) at least one split intein domain; a second vector having a second donor sequence that encodes (i) a second non-native protein and (ii) at least one split intein domain. The respective split intein domains encoded by the first and second vectors are configured to associate with one another and ligate the first and second non-native proteins to thereby form a fused protein.
3.20220017918-SYNTHESIS OF NON-NATIVE PROTEINS IN BOMBYX MORI BY MODIFYING SERICIN EXPRESSION US - 20.01.2022 Int.Class C12N 15/85 Appl.No 17377318 Applicant Kraig Biocraft Laboratories, Inc. Inventor Kimberly Kraig Thompson Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with sericin proteins. Embodiments include vectors utilized for modifying one or more sericin genes. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into a targeted sericin gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing a non-native protein product with minimized or prevented production of sericin.
4.20220017917-SYNTHESIS OF HIGH MOLECULAR WEIGHT PROTEINS USING INTEINS US - 20.01.2022 Int.Class C12N 15/85 Appl.No 17377312 Applicant Kraig Biocraft Laboratories, Inc. Inventor Kimberly Kraig Thompson This disclosure is directed to split intein protein production systems using transgenic target organisms such as Bombyx mori. A vector set for transforming a target organism includes: a first vector having a first donor sequence that encodes (i) a first non-native protein and (ii) at least one split intein domain; a second vector having a second donor sequence that encodes (i) a second non-native protein and (ii) at least one split intein domain. The respective split intein domains encoded by the first and second vectors are configured to associate with one another and ligate the first and second non-native proteins to thereby form a fused protein.
5.20190185528-TRANSGENIC SILKWORMS CAPABLE OF PRODUCING CHIMERIC SPIDER SILK POLYPEPTIDES AND FIBERS US - 20.06.2019 Int.Class C07K 14/435 Appl.No 16275159 Applicant University of Notre Dame du Lac Inventor Malcolm James Fraser Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.
6.20150322121-CHIMERIC SPIDER SILK POLYPEPTIDES AND FIBERS AND USES THEREOF US - 12.11.2015 Int.Class C07K 14/435 Appl.No 14754916 Applicant THE UNIVERSITY OF NOTRE DAME Inventor Malcolm James FRASER Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.
7.20190153047-TRANSGENIC SILKWORMS CAPABLE OF PRODUCING CHIMERIC SPIDER SILK POLYPEPTIDES AND FIBERS US - 23.05.2019 Int.Class C07K 14/435 Appl.No 16246318 Applicant University of Notre Dame du Lac Inventor Malcolm James Fraser, Jr. Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.
8.20210246471-MODIFICATION OF HEAVY CHAIN FIBROIN IN BOMBYX MORI US - 12.08.2021 Int.Class C12N 15/90 Appl.No 17172818 Applicant Kraig Biocraft Laboratories, Inc. Inventor Trevor Logan Kane Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with the heavy chain fibroin protein. Embodiments include insertion and truncation vectors utilized for modifying the FibH gene. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into the FibH gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing an enhanced silk product with a high percentage of spider silk proteins. Embodiments include a silk product produced by such transgenic Bombyx mori.
9.WO/2021/163249-MODIFICATION OF HEAVY CHAIN FIBROIN IN BOMBYX MORI WO - 19.08.2021 Int.Class C12N 15/90 Appl.No PCT/US2021/017544 Applicant KRAIG BIOCRAFT LABORATORIES, INC. Inventor KANE, Trevor Logan Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with the heavy chain fibroin protein. Embodiments include insertion and truncation vectors utilized for modifying the FibH gene. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into the FibH gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing an enhanced silk product with a high percentage of spider silk proteins. Embodiments include a silk product produced by such transgenic Bombyx mori.
10.20150322122-TRANSGENIC SILKWORMS CAPABLE OF PRODUCING CHIMERIC SPIDER SILK POLYPEPTIDES AND FIBERS US - 12.11.2015 Int.Class C07K 14/435 Appl.No 14754946 Applicant THE UNIVERSITY OF NOTRE DAME Inventor Malcolm James FRASER Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.