Replies to post #127190 on PharmaCyte Biotech Inc (PMCB)

[1234jklm]

New England journal of Medicine doesn’t publish unverified claims.

Cited 9 times

https://www.nejm.org/doi/full/10.1056/NEJM200404083501523

Boosting the Sensitivity of Real-Time Polymerase-Chain-Reaction Testing for SARS
9 Citing Articles
TO THE EDITOR:
In view of recent concern about the recurrence of severe acute respiratory syndrome (SARS) in Guangdong, China, we would like to highlight the tremendous importance of sensitivity in testing for the SARS-associated coronavirus (SARS-CoV). Because the initial symptoms of SARS are similar to those of other common respiratory diseases, making a specific diagnosis of SARS poses difficulties to medical professionals. Our enhanced real-time (ERT) polymerase-chain-reaction (PCR) method (first presented in June 2003 at a symposium on SARS1) has been designed for the detection of SARS-CoV with high sensitivity and easy-to-interpret results.2 The power of the ERT technique has now been extensively explored with the development of ERT-based diagnostic tests for various infectious diseases, including avian influenza and foot-and-mouth disease.
Figure 1.

Results of Modified Enhanced Real-Time (ERT) Polymerase Chain Reaction (PCR) with One-Step Reverse-Transcriptase PCR (RT-PCR).
Since the first report of ERT results for SARS,2 the ERT technique has been modified to increase its sensitivity for the detection of SARS-CoV by at least 10 times (Figure 1). This improved sensitivity has been achieved by combining the reverse-transcriptase (RT) and PCR steps into a single step (described in Supplementary Appendix 1, available with the full text of this letter at www.nejm.org). In addition, the procedural change makes the diagnostic procedure more convenient. These salient features of one-step RT-PCR have thus far been overlooked by other researchers in this field. Because the single RT-PCR step and the subsequent real-time PCR step require only 35 cycles, the detection of SARS-CoV by the modified ERT technique yields results quickly and with higher sensitivity than regular real-time PCR assays reported to date.
As noted by the World Health Organization with respect to the shortcomings commonly seen in available diagnostic tests for SARS,3 it is important to unify a molecular test for SARS that can provide sensitive, reliable, and accurate results. Currently, many research groups claim that their methods are accurate, but the way in which they evaluate accuracy is not clearly described.4 The usefulness of an accurate test that lacks sensitivity has yet to be determined. Unless a unified molecular test for SARS with high sensitivity and reliability is available, we may face the risk of false negative test results, which would allow infected patients to slip into the community and avoid control measures set up to isolate carriers.
Over a year after the start of the 2003 SARS outbreak, many people are still struggling to recover from the physiological and psychological scars inflicted at that time. Identifying potential SARS-CoV carriers by a method with high sensitivity and reliability and as early as possible is crucial to avoid a repetition of the 2003 outbreak.
Albert Cheung-Hoi Yu, Ph.D.
Peking University, Beijing 100083, China
[e-mail]
Lok-Ting Lau, Ph.D.
Yin-Wan Wendy Fung, Ph.D.
Hong Kong DNA Chips, Hong Kong, China

[DragonBear]

just because an inventor says his invention is the best doesn’t make it so.

Say it ain't so! It's easy enough to lookup how sensitive the RT-PCRs already in the market are. Matter of public record in the EUA forms. But for the Hai Kang kits one just has to take Yu's word on it.

People need to get real. It’s just another dog that doesn’t hunt.

Maybe reading Kenny PR pumps less, and learning about the Covid virus more would help? Such limited space for reality.

There is so much competition out there that’s way ahead of them already.

As in there are other RT-PCRs in the US market that works just fine. In fact they have successfully been sensitive enough to work in other countries for "pooled" samples.

Yet we have the pretending that the whole world or at least the US is waiting for the Hai Kang kits to be validated by Kenny et al. Yep, Hai Kang kits are going to save the world from Covid.

Why not! It follows the script. After all the Kenny Wonder Treatment is going to save the world from PC and a whole host of other solid tumor cancers.