Replies to post #189659 on Kraig Biocraft Laboratories Inc (KBLB)

[first mike]

Hi imelcooler,
you asked: "what thoughts you may have specifically in the area of science
and as it relates to Knock in Knock out in relation to past methods
in particular "CRISPER Cas9"?"

KBLB had its first big "knock-out / Knock-in success several years ago using Zinc Fingers CompoZr (see: "The Platform Worm").
But the problem with Zinc Fingers is that it is Expensive, requiring the purchase of each different vector-selector from a third party (Sigma Aldritch-Sangamo). This also slowed and stifled ingenuity. The advantage of the closely held generator technology was that we had exclusive rights in our field.

CRISPER Cas9 has been the darling prodigal son for the last couple of years but I expect that is mostly because it is Cheap and Easy.
It was developed in a College lab and essentially given to the world.
Most college labs can duplicate and use it. But it is not, to my knowledge any better at selectivity than Zinc Fingers, and we lost our IP exclusivity! There is another new splicing tech out there called TALENS but at this time it seems to be an "Also ran".

This newest press conference announcement seems to imply that KBLB has perfected its own highly successful proprietary splicing technology.
This in itself could be a very valuable Intellectual Property.

"Pure Spider Silk" is a nonsensical currently popular buzz-word.
There is no such thing.
What the press conference was describing though was success in creating arbitrary genetic mixtures of integrated silk components, a goal long sought after by KBLB.

The original chimeric silk described in the landmark PNAS paper was only about 5 to 10% spidroin tightly integrated into the structure of silkworm silk.
The breakthrough was that it WAS tightly integrated into the structure of the silk. This allowed that small percentage to almost double the tensile strength of the silk.
BTW, we owe that breakthrough to the genius level insight into silk genetics of Dr. Davis, one of KBLB's early contributors.
He designed the synthetic precursor sequence that allowed that integration.
THAT was what distinguished KBLB's process from many failed predecessors.

The 2020 press conference announcement was that we could now incorporate much higher levels of spidroin, presumably leading to much stronger silks and to silks with tailored properties as we desired.

Mike L.