News 
Market Data 
Discover
Replies to post #8804 on SiNtx Technologies Inc (SINT)
These peculiar phases were found to be particularly efficient in stimulating the in vitro differentiation of KUSA-A1 cells into osteoblasts, although according to different microscopic mechanisms. The final amount of bone formation was nearly identical for both phases. Cell differentiation was monitored by assessing the concentration of the osteogenic marker ?-carboxyglutamate (i.e., Gla-osteocalcin). It was found to be ~45% higher for Si3N4 samples possessing the N-apatite phase than for biomedical titanium alloy controls tested under exactly the same conditions. Concurrent measurements of the bone resorption marker Glu-osteocalcin (i.e., an undercarboxylated form of ?-carboxyglutamate) showed significant inhibition of osteoclastogenesis on these surface-treated Si3N4 samples as compared to the controls. Bone formation was assessed using in situ Raman microprobe spectroscopy and ex situ laser microscopy. These two independent analytical techniques consistently found an increase of ~80% in expressed hydroxyapatite when compared to a biomedical titanium alloy. This study suggests that surface-treated Si3N4 may have a powerful anabolic, differentiating, and antiapoptotic effect on osteoblasts in vitro, and a concurrent inhibitive action on osteoclastogenesis. Given additional research, Si3N4 may represent a new therapeutic solution for bone disorders and for engineered implants that physiologically regulate bone growth processes.
