Replies to post #101804 on Micro Imaging Technology Inc (MMTC)

[Dreamchaser]

Great Job, MMTC

Staphylococcus aureus is the most commonly isolated human associated bacterial pathogen. It plays an important role in skin and soft-tissue infections, pneumonia, endocarditis, osteomyelitis, foreign-body infections, and sepsis. S. aureus diagnosis and treatment requires a minimum of 24-48. With this in mind, previous studies suggest that faster pathogen identification has been linked to improved patient outcomes. Improved patient outcomes including a reduction in hospitalization time, decreased risk of nosocomial infections and decreased in medical costs. The impact of faster identification on patient outcome has led us to develop an alternative method of S. aureus identification via ImmunoMagnetic Separation (IMS) and laser-light scattering identification technology. With this method, we hypothesized that anti-Protein A conjugated to magnetic DynaBeads could bind to surface Protein A on S. aureus from swab sample and facilitate their isolation upon exposure to a magnetic field within a 4-8 hour procedure. S. aureus cells isolated by IMS would then be accurately identified using laser-light scattering technology in less than 5 minutes. MIT identification accuracy analysis was conducted and displayed that both laboratory and clinical Staphylococcus species strains identified at a rate greater than 95% and negative control strains identified at a rate less than 1%. Our developed methods displayed statistically significant (P < 0.001) specificity for S. aureus and capture efficiency greater than 80%. The combination of IMS and laser-light identification gives a rapid and accurate identification in less than 8 hours, which is significantly less than traditional culture-based identification methods.

http://commons.nmu.edu/theses/84/

[Dreamchaser]

The Direction of Micro-Imaging Technologies, Inc

It might as well be a press release since Hollen is now employed by MMTC in their private lab.

In the future we also plan to perform IMS capture, enrichment, and laser-light scattering identification on patient swab samples obtained directly from infected wounds or soft tissue infections. We hope to perform a single-blind experiment, similar to the single-blind experiment conducted in chapter two, with the addition of IMS capture and enrichment directly from patient swab specimens.Future directions in combination with our collaborator, MIT, include improving the Staphylococcus species Identifier to specifically identify Staphylococcus aureus. Currently MIT reports that the S. aureus Identifier is 95% completed. Once the S. aureus Identifier is complete the S. aureus Identifier will be validated by testing in the Sharp lab.
We have over two dozen different S. aureus isolates in our strain collection to test the strength of this Identifier. MIT is also making initial light scattering measurement on a MRSA strains. We purchased two MRSA panels from the American Type Collection that contains 17 different MRSA strains with different sccMec types. It will be interesting to
determine if MRSA strains have any consistent light scattering pattern differences compared to methicillin-sensitive S. aureus (MSSA) strains. The mecA gene genomic insertions that confer methicillin resistance are relatively small, between 2.820-2.903 Mb nucleotides and the protein content of the MRSA cell is likely very similar to MSSA strains. It may push the limits of the technology to differentiate MRSA from MSSA. MIT also plans to automate the methods we have generated. Although this system generates an identification for S. aureus faster than traditional methods it is still fairly labor intensive.

http://commons.nmu.edu/cgi/viewcontent.cgi?article=1107&context=theses

[cottonmather]

mmtc BS galore. mmtc longs were promised certs for E Coli and Salmonella for spring of 2014 - none ever came after three failed attempts. Then their direction turned to greater libraries - none were ever seen. Then mmtc promised faster test preps and no one has ever seen them. And then there was the "poster award" at a 2nd rate school that meant nothing. And now a goofball "thesis paper".

Do you know what a "thesis paper" is? It is an explanation of a guess. It proves nothing. You can put all the fancy language in it that you want and it means absolutely nothing. It's just BS just like everything else mmtc says. It never happens. It's not true. By design it is to round up schmucks and make them bagholders. That is the business of mmtc - sell stock to fools. mmtc SUCKS BIG TIME

[cottonmather]

mmtc BS galore. mmtc longs were promised certs for E Coli and Salmonella for spring of 2014 - none ever came after three failed attempts. Then their direction turned to greater libraries - none were ever seen. Then mmtc promised faster test preps and no one has ever seen them. And then there was the "poster award" at a 2nd rate school that meant nothing. And now a goofball "thesis paper".

Do you know what a "thesis paper" is? It is an explanation of a guess. It proves nothing. You can put all the fancy language in it that you want and it means absolutely nothing. It's just BS just like everything else mmtc says. It never happens. It's not true. By design it is to round up schmucks and make them bagholders. That is the business of mmtc - sell stock to fools. mmtc SUCKS BIG TIME