Replies to post #12950 on Inovio Pharmaceuticals Inc (INO)

[Ubertino]

I see no problem with electroporation - simple, works effectively, well tested.

Ultrasound is also available:

Cell Biol Toxicol. 2010 Feb;26(1):21-8. doi: 10.1007/s10565-009-9144-8. Epub 2009 Dec 1.

Electroporation and ultrasound enhanced non-viral gene delivery in vitro and in vivo.

Wells DJ1.
Author information
1Department of Cellular and Molecular Neuroscience, Imperial College London, UK. [e-mail]

Abstract
Non-viral vectors are less efficient than the use of viral vectors for delivery of genetic material to cells in vitro and especially in vivo. However, viral vectors involve the use of foreign proteins that can stimulate both the innate and acquired immune response. In contrast, plasmid DNA can be delivered without carrier proteins and is non-immunogenic. Plasmid gene delivery can be enhanced by the use of physical methods that aid the passage of the plasmid through the cell membrane. Electroporation and microbubble-enhanced ultrasound are two of the most effective physical delivery methods and these can be applied to a range of different cell types in vitro and a broad range of tissues in vivo. Both techniques also have the advantage that, unlike viral vectors, they can be used to target specific tissues with systemic delivery. Although electroporation is often the more efficient of the two, microbubble-enhanced ultrasound causes less damage and is less invasive. This review provides an introduction to the methodology and summarises the range of cells and tissues that have been genetically modified using these techniques.

http://www.ncbi.nlm.nih.gov/pubmed/19949971

[957Df]

Better profile stability at room temperature compared to Conventional Vaccines

Sorry, your opinion is a big mistake. I do not know where did you get your information, but the vaccines have a great advantage because they are thermally stable and do not require refrigeration as conventional vaccines. Go to their website and read the content again:

"They have a better profile stability at room temperature compared to Conventional Vaccines and May and not require cold-chain for storage and transport. These Important Advantages yield more Timely vaccine development and can Improve accessibility in nations with less Developed infrastructure"