Replies to post #110752 on Amarantus Bioscience Holdings Inc (AMBS)

[GS1]

Read the paper below from Dr. Kriby.

http://content.stockpr.com/amarantus/files/pdf/Poster+2+-+Clinical+FINAL_v2.pdf

Some excerpts from the link..

Assay methods:
Sample preparation: Sample preparation for [1] and [2] were as published and similar. In brief, peripheral blood mononuclear cells (PBMC) were isolated from whole heparinized blood samples as described previously [1]. Aliquots of each sample were stimulated with either phytohemaglutinin (PHA, 12 µg/mL), pokeweed mitogen (PWM, 4 µg/mL) or none (Unstimulated) and each cell culture incubated at 37°C with 7% CO2 for four hours [1] and [2] (Version 1 = V1).

The enhanced version (Version 2 = V2) included 8 µg/mL PWM with up to 20 hours incubation.Samples were then diluted and frozen prior to staining for flow cytometry.