Pacific Biosciences of California Inc (PACB)

[Paulieme]

Join the PacBio® team as we discuss the recently released human MCF-7 Iso-Seq transcriptome dataset. The webinar will cover both the bioinformatics pipeline that was used in generating the data and explore the pipeline results.
•Bioinformatics Pipeline?
?Identification of full-length reads using polyA tail signal and adapters
?Iterative clustering of reads at the isoform-level
?Final consensus clustering using Quiver
•Pipeline Results
?Novel transcripts
?Alternative splice forms
?Alternative polyadenlyation
?Fusion genes
Who should attend:
Researchers and bioinformaticians interested in full-length transcriptome sequencing.

Recommended pre-reading:
•Full-length cDNA sequencing protocol
•Basic understanding of PacBio sequencing data format
Presenter BiographyElizabeth Tseng
Senior Bioinformatics Scientist, Pacific Biosciences

Elizabeth obtained her doctorate degree in Computer Science & Engineering from the University of Washington in 2012. Her thesis work focused on the computational discovery of bacterial non-coding RNAs and gut microbiome. After joining PacBio, she decided to give prokaryotes a break and now supports and develops eukaryotic transcriptome-related collaborations.
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time below to register:
Wednesday, January 22 8:00 a.m. PST

Wednesday, January 22 5:00 p.m. PST
http://programs.pacificbiosciences.com/l/1652/2013-12-06/2tw4q9