Wednesday, November 06, 2013 3:53:09 PM
Posted 06 Nov 2013 Using PacBio and Illumina whole genome shotgun sequences we recently released for the D. melanogaster reference strain, Sergey Koren and Adam Phillippy at the University of Maryland have recently run their pacBioToCA method to generate a dataset of error-corrected PacBio reads for this dataset, which the have kindly made available here for re-use without restriction. This pilot data set is not at high enough coverage and thus a whole genome assembly was not attempted. Nevertheless, both the raw and error-corrected datasets should be of use to better understand the nature of PacBio data and the pacBioToCA pipeline as applied to Drosophila genomes.
The 2057_PacBio_corrected.tgz archive contains the following files:
•pacbio.blasr.spec – the specification file used for the pacBioToCA run.
•corrected.trim.fastq.bz2 – the error-corrected PacBio reads.
•corrected.trim.lens – a file containing columns with corrected read id, read length, running total BP, running mean, and running coverage assuming 140Mbp genome size.
•corrected.trim.names – a look-up table to map read IDs in the pacBioToCA output to the original PacBio input IDs.
Sergey also sent along a quick summary of the correction:
The correction used the latest CA from the repository (as of 10/15/13). The max number of mappings each Illumina sequence could have was set to 20, the repeat separation was set to 10. The genome size was set to 180Mbp. BLASR was used to align the Illumina sequences to PacBio sequences for correction. The input for correction was 673 Mbp of PacBio data (max: 8,231, mean: 1,587) which corresponds to approximately 4.8X of a 140Mbp genome. The correction produced 469Mbp (max: 6,883, mean: 1,410) or 3.35X. The throughput was approximately 69%. To estimate, the accuracy of the data, it was mapped back to the reference D. melanogaster. The sequences average 99.85% identity. Approximately 10% of the sequences mapped in more than a single piece to the reference. Some of these mappings were due to short indel differences between the reference and the reads or N’s in the reference. Some sequences mapped across large distances in the reference. I did not confirm if these split mappings were supported by the uncorrected reads or not.
We thank both Sergey and Adam for taking the time to run their pacBioToCA pipeline and making these data available to the community, and hope these data are of use to others in their research.
http://bergmanlab.smith.man.ac.uk/?p=2151#.UnqOzFOzu7I.twitter
Recent PACB News
- Ambry Genetics and PacBio Announce Collaboration to Sequence Up to 7,000 Human Genomes Aimed at Providing Answers for Families Battling Rare Diseases • PR Newswire (US) • 05/15/2024 01:45:00 PM
- Form S-3ASR - Automatic shelf registration statement of securities of well-known seasoned issuers • Edgar (US Regulatory) • 05/09/2024 08:33:12 PM
- Form 10-Q - Quarterly report [Sections 13 or 15(d)] • Edgar (US Regulatory) • 05/09/2024 08:21:46 PM
- Form 8-K - Current report • Edgar (US Regulatory) • 05/09/2024 08:12:15 PM
- PacBio Announces First Quarter 2024 Financial Results • PR Newswire (US) • 05/09/2024 08:05:00 PM
- PacBio Announces Preliminary First Quarter 2024 Revenue and Updates 2024 Revenue Guidance • PR Newswire (US) • 04/16/2024 12:05:00 PM
- Estonia National Biobank Selects PacBio to Sequence 10,000 Whole Genomes • PR Newswire (US) • 03/27/2024 12:00:00 PM
- PacBio Grants Equity Incentive Award to New Employee • PR Newswire (US) • 03/22/2024 08:30:00 PM
- PacBio Announces PureTarget™ Repeat Expansion Panel, Expanding its Portfolio of End-to-End Clinical Research Solutions • PR Newswire (US) • 03/12/2024 01:05:00 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 03/06/2024 10:36:07 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 03/06/2024 10:30:18 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 03/06/2024 10:26:40 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 03/06/2024 10:22:45 PM
- Form 144 - Report of proposed sale of securities • Edgar (US Regulatory) • 03/04/2024 11:32:39 PM
- Form 144 - Report of proposed sale of securities • Edgar (US Regulatory) • 03/04/2024 11:22:32 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 02/26/2024 09:55:28 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 02/26/2024 09:36:09 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 02/26/2024 09:25:48 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 02/26/2024 09:19:42 PM
- PacBio to Present at Upcoming Investor Conferences • PR Newswire (US) • 02/26/2024 09:05:00 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 02/21/2024 11:25:13 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 02/21/2024 11:20:57 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 02/21/2024 11:17:14 PM
- Form 4 - Statement of changes in beneficial ownership of securities • Edgar (US Regulatory) • 02/21/2024 11:07:18 PM
- Form 144 - Report of proposed sale of securities • Edgar (US Regulatory) • 02/20/2024 09:17:12 PM
Greenlite Ventures Inks Deal to Acquire No Limit Technology • GRNL • May 17, 2024 3:00 PM
Music Licensing, Inc. (OTC: SONG) Subsidiary Pro Music Rights Secures Final Judgment of $114,081.30 USD, Demonstrating Strength of Licensing Agreements • SONGD • May 17, 2024 11:00 AM
VPR Brands (VPRB) Reports First Quarter 2024 Financial Results • VPRB • May 17, 2024 8:04 AM
ILUS Provides a First Quarter Filing Update • ILUS • May 16, 2024 11:26 AM
Cannabix Technologies and Omega Laboratories Inc. enter Strategic Partnership to Commercialize Marijuana Breathalyzer Technology • BLO • May 16, 2024 8:13 AM
Avant Technologies to Revolutionize Data Center Management with Proprietary AI Software Platform • AVAI • May 16, 2024 8:00 AM