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es1

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es1

Re: downsideup post# 54987

Thursday, 03/14/2013 10:46:18 PM

Thursday, March 14, 2013 10:46:18 PM

Post# of 276084
So lets put this to rest

That doesn't begin to address the issue with a resulting lack of uniformity and variation in strength within a fiber... when uniformity and consistent strength are important factors you consider in the utility of any fiber, along with consistent repeat-ability in the production of fibers with some demonstrated uniformity between them.



I am curious where you get the "lack of uniformity" from.

Apparently you think the national academy of science is not as smart as you? Do you really think the scientists there dont know that there are ALWAYS variables and that they only checked a single 19mm strand of silk?
You actually think that they are stupid enough to think that any piece of fiber would represent the whole?
They tested between 7 and 19 strands of each cocoon. They were all pretty consistent even between each different strains

But let me help you out...


Preliminary data had shown that this
relatively large synthetic spider silk protein could be spun into
fibers by extrusion after being produced in E. coli, indicating that
it had the inherent ability to assemble into fibers. However, we also recognized that this protein would be expressed in concert
and would have to interact with the endogenous silkworm fhc,
fibroin light chain, and fibrohexamerin proteins to be incorporated
into silk fibers
. Thus, we embedded the A2S814 spider
silk sequence within N- and C-terminal fhc domains to direct the
assembly process, as originally described by Kojima
and coworkers


Now why would that help you??? well read the referenced paper and you will find...

The cocoons of KH25 showed fluorescence(Fig3G)This means that HC-EGFP protein was spun out without inhibition of the normal spinning process,and that HC-EGFP protein retained its conformation after spinning. This suggests that any other foreign protein fused into modified H-chain protein will be produced consistently with its native structure and will bring its native characteristics to the silk fiber of the transgenic silkworm.



The silk made by KBLB is consistent and is in all ways superior to silkworm silk and Amsilks fibers.
The beginning of the first quote is what your friends at Amsilk make for $100K a kilo from ecoli goo and what R Lewis tried with goat goo.

So your "problem" is now no longer valid... Time to move on to the next "problem". What do you got left?

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