Secondly, they are pluripotent as will be the single blastomere lines developed through ACT. If the single blastomere lines NED 1-4 were not pluripotent why would the NIH be trying to include them in this definition?
Therefore, the NIH proposes replacing the current definition of hESCs in Section II with the following: ‘‘For the purpose of these Guidelines, ‘human embryonic stem cells (hESCs)’ are pluripotent cells that are derived from early stage human embryos, up to and including the blastocyst stage, are capable of dividing without differentiating for a prolonged period in culture, and are known to develop into cells and tissues of the three primary germ layers.’’
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